Conducting Auxiliary Clinical Diagn Rapid Test Kits Detecting Sperm Count Human Semen In Vitro

The kit is used for qualitatively detecting sperm count of human semen in vitro, and conducting auxiliary clinical diagnosis CE certified

Applications: 

The kit is used for qualitatively detecting sperm count of human semen in vitro, and conducting auxiliary clinical diagnosis of the infertility and self-evaluation male fertility of pre-pregnancy.

Description: 

The kit for sperm count detection (Colorimetric method) used the inert glass fiber membrane with high water absorption and the pore size of less than 0.5um to filter the semen, and sperm cells will be trapped on surface of the first layer of the membrane, and we use the staining fluid that can quickly dye sperm cells, the darker of the color, the more of sperm count on the filter membrane, and the count of sperm in the semen will be higher. Through comparing with the reference color (20million/ml specified by WHO) of well B on the test board, we know that if color of the test well A is lighter than color of the color card, the sperm count is positive, and if color of the test well A is darker than the color card, the sperm count is negative.

How to use? 

Before testing, we must read the instructions completely first

1. Specimen collection of semen: Using masturbation to expel the semen directly into the sperm collecting cup, or using a condom to collect semen during the sex life, to pour all the semen into the semen collecting cup.

2. Liquefaction: To shake evenly the semen in the semen collecting cup, and standing it for 15 minutes, until the semen becomes fluid liquid.

3. Add the specimen: To take out an experimental board from a aluminum foil bag, and place it on the operation platform horizontally, using an accessory specimen-absorption pipe to absorb a tube of semen from the semen collecting cup, and dropping three drops of the semen into the white A hole, and then the semen saturates to surface of the membrane and dry.

4. Add blue staining fluid: To add one drop of blue staining solution to test well A , and let it soak for 1-2 minutes.

5. Add transparent washing buffer: Add two drops of transparent washing buffer to test well A, and let it soak for 1-2 minutes, and then read the results immediately.

6. Read the results: Read the color of test well A , compare colors of test well A to reference Well B, the darker of the color, the more of sperm count, and report the results.

INTERPRETATION OF RESULTS

(Please refer to the illustration above)

Results of naked eye observation: Compare colors of test well A to reference Well B.

Negative reactive results: The color of test well A is darker than standard color of reference Well B, and which shows that count of sperm ≥20million/ml, and count of the sperm is normal.

Positive reaction results: The color of test well A is lighter than standard color of reference Well B, and which shows that count of the sperm≤20million /ml, such case belongs to oligospermatism (5million/ml≤ count of the sperm≤20million/ml); If well A is colorless, and which shows that count of the sperm≤5million/ml, such case belongs to severe oligozoospermia or azoospermia.

The subject whose test result is positive or can not be judged easlily shall be repeated the test in well C, the testing result of well A shall be the same with well C. If the results are different, and the correction test shall be conducted in well D (to add 4 drops of semen into well D), and the results can be explained as the following table. The subject shall be repeatedly examined in 3-7 days after the first test, and consult male doctors about the obtained results.