AllTest Antibodies To Tetanus Rapid Test Kits Home Professional Use ITE-402

AllTest Antibodies To Tetanus Toxin Rapid Test Kits Home Professional Use ITE-402

Applications: 

TETANUS  is   an   immunochromatographic  rapid   test   for  semi-quantitative  detection  of anti  tetanus  toxin  antibodies  in  serum,  plasma or  human whole blood.TETANUS  is intended  for professional  use within  the framework of laboratory analysis or point of care analysis to detect immunity to tetanus toxin.

Description: 

Clostridium tetani is a bacterium that causes tetanus in humans. Clostridium tetani ate Gram-positive, spore-forming rods that are anaerobic. If they enter the body through a wound, they can multiply and produce a toxin that affects the nerves and controls the activity of muscles. Toxin of Clostridium tetani binds to membranes of peripheric nervous cells and inhibits the release of neurotransmitters.

Antibodies to tetanus toxin are produced in the human by the injection of chemically inactivated tetanus toxin (tetanus toxoid). Immunization is the best way to prevent C. Tetani infections in children and adults. Moreover, injection of specific and purified anti tetanus toxin IgG is used in order to refrain toxin action during an acute infection.

It is sometimes better to know the level of anti tetanus toxin antibodies in a patient, to evaluate their immune status, in order to determine the necessity of a complementary vaccination which would assure immunity towards tetanus toxin.

In emergency situations, it is important for the clinician to know the immune status in order to decide on the correct anti-tetanus prophylaxis for high risk patients (deep wounds).

How to use? 

Allow the test, specimen, buffer and/or controls to reach room temperature (15-30°C) prior to testing.

1. Bring the pouch to room temperature before opening it. Remove the test cassette from the sealed pouch and use it as soon as possible.
2. Place the cassette on a clean and level surface.

For Serum or Plasma specimen:

• Hold the dropper vertically and transfer 1 drop of serum or plasma (approximately 25 mL) to the specimen well of test cassette, then add 3 drops of buffer (approximately 120ul), and start the timer. See illustration below.

For Venipuncture Whole Blood specimen:

• Hold the dropper vertically and transfer 1 drop of whole blood (approximately 25 mL) to the specimen well, then add 3 drops of buffer (approximately120 mL), and start the timer. See illustration below.                                                                                                                         3.Wait for the colored line(s) to appear. Read results at 10 minutes.  Do not interpret the result after 20 minutes

INTERPRETATION OF RESULTS

(Please refer to the illustration above)

 POSITIVE:* Two distinct colored lines appear. One colored line should be in the control region (C) and another colored line should be in the test region (T). Sample is considered positive with an antibody level above 0.1 IU/ml.

*NOTE: The intensity of the color in the test line region (T) will vary depending on the concentration of Tetanus antibody present in the specimen. Therefore, any shade of color in the test region (T) should be considered positive.

NEGATIVE: One colored line appears in the control region (C). No apparent colored line appears in the test region (T). (with anti  tetanus antibody  level below  0.1 IU/ml).

INVALID: Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test with a new test cassette. If the problem persists, discontinue using the test kit immediately and contact your local distributor.