High quality Rapid Test Kits Accuracy Canine CRP Test
cCRP Antigen Rapid Test Cassette (Whole Blood/Serum/Plasma) is a sandwich lateral flow immunochromatographic assay for the
semi-quantitative detection of CRP antigen in canine’s whole blood and serum or plasma.
cCRP Antigen Rapid Test Cassette is based on lateral flow immunochromatographic assay. The test cassette has a testing window. The testing window has an invisible T (test) zone and C (control) zone. When sample is applied into the sample well on the cassette, the liquid will laterally flow on the surface of the test strip. If there is enough CRP antigen in the sample, a visible T line will appear. The C line should always appear after a sample is applied, indicating a valid result. By this means, the cassette can accurately indicate the presence of CRP antigen in the sample.
How to use?
Allow the test cassette, specimen, buffer, and/or controls to equilibrate to room temperature (15-30 °C) prior to testing.
1. Collect sample
Collect canine’s fresh whole blood, or separate serum or plasma from blood as soon as possible to avoid hemolysis. Serum and plasma specimens may be stored at 2-8 °C for up to 3 days. For long term storage, serum and plasma specimens should be kept below -20°C. Whole blood should be stored at 2-8°C if the test is to be used within 2 days of collection. Do not freeze whole blood specimens.
2. Sample Dilution The 10μL of blood specimen or 5μL serum or plasma can be added directly with the capillaries into the plastic tubes with buffer. Please note that the blood-filled end-to-end capillary is 10μL. Close the tube and shake the sample by hand forcefully for approximately 10 seconds so sample and dilution buffer mix well.
3. Place the test cassette on a clean and level surface. Hold the dropper vertically and transfer the 2 drops of Diluted specimen (approximatiely 50μL) to the specimen well (S) of the test cassette, then start the timer. See illustration below.
4. Read the results in 5-10 minutes by comparing the T line intensity with the color card provided. Do not interpret results after 15 minutes.
INTERPRETATION OF RESULTS
CRP, as an acute phase protein in vivo, is a normal component with a low level in the blood. When there are bacterial infections, active lesions
and extensive tissue damage in vivo, CRP is rapidly increased. When CRP is more than 10mg/L, it indicates infection, mild and moderate
bacterial infection usually has only a low or moderate level of elevation, and more than 50mg/L indicates widespread infection.
The presence of “C” line indicates a valid result. The CRP test can be run in between 1 μg/mL and 200 μg/mL. Read the result by
comparing the color intensity of the “T” line with the standard color card provided.
Positive: The presence of both “C” line and zone “T” line. T line at G3 or G4 is considered as positive.
Negative: The presence of both “C” line and zone “T” line. T line at G1or G2 is considered as negative.
Invalid:No colored line appears in C zone, regardless of T line’s appearance.