High Sensitivity Cryptosporidium Test Kit Diagnostic Cassette Lateral Flow Test Strips

A rapid test for the qualitative detection of Cryptosporidium​ in human Feces samples CE certified

Applications:

The Cryptosporidium Antigen Rapid Test Cassette (Feces) is a rapid chromatographic immunoassay for the qualitative detection of Cryptosporidium Antigens in human feces.

Description:

Cryptosporidiosis is a diarrhoeal disease caused by microscopic parasites of the genus Cryptosporidium. Once an animal or person is infected, the parasite lives in the intestine and passes in the stool. The parasite is protected by an outer shell that allows it to survive outside the body for long periods of time and makes it very resistant to chlorine-based disinfectants. Both the disease and the parasite are commonly known as "Crypto." The disease can spread through ingestion of contaminated water or through coughed fomites of an infected individual. It can spread by fecal-oral route like other gastrointestinal pathogens.

How to use?

DIRECTIONS FOR USE
1.To process fecal specimens:For Solid Specimens:
Unscrew the cap of the specimen collection tube, then randomly stab the specimen collection applicator into the fecal specimen at least 3 different sites to collect approximately 50 mg of feces (equivalent to 1/4 of a pea). Do not scoop the fecal specimen.
For Liquid Specimens:
Hold the dropper vertically, aspirate fecal specimens, and then transfer 2 drops of the liquid specimen (approximately 80 µL) into the specimen collection tube containing the extraction buffer. Tighten the cap onto the specimen collection tube, then shake the specimen collection tube vigorously to mix the specimen and the extraction buffer. Leave the collection tube for reaction for 2 minutes.
2. Bring the pouch to room temperature before opening it. Remove the test cassette from the foil pouch and use it as soon as possible. Best results will be obtained if the test is performed immediately after opening the foil pouch.
3. Hold the specimen collection tube upright and unscrew the tip of the specimen collection tube. Invert the specimen collection tube and transfer 3 full drops of the extracted specimen (approximately 120µL) to the specimen well (S) of the test cassette, then start the timer. Avoid trapping air bubbles in the specimen well (S). See illustration below.
4. Read the results at 10 minutes. Do not interpret the results after 20 minutes.
Note: If the specimen does not migrate (presence of particles), centrifuge the diluted sample contained in the extraction buffer vial. Collect 120 µL of supernatant, dispense into the specimen well (S) of a new cassette. Start the timer and continue from step 4 onwards in the above instructions for use.

INTERPRETATION OF RESULTS

POSITIVE: Two lines appear. A red colored line appears in the Test line region (T) and another line appears in the C line region (C).
*NOTE: The intensity of the color in the test line region (T) will vary depending on the concentration of antigens present in the specimen. However, neither the quantitative value, nor the rate of increase in antigens can be determined by this
qualitative test.
NEGATIVE: Only one line appears in control line region (C). No line appears in the test line region (T).
INVALID: Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test with a new test. If the problem persists, discontinue using the test kit immediately and contact your local distributor.