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High Quality And Convenient Professional Rotavirus / Adenovirus Combo Test Cassette

Product Details:

Place of Origin:CHINA
Brand Name:AllTest
Certification:CE
Model Number:CASSETTE

Payment & Shipping Terms:

Minimum Order Quantity:500
Packaging Details:25 tests per kit
Supply Ability:100 Million a year
Detailed Product Description
Format: Cassette Color: Blue
Specimen: Feces Storage: 2-30°C
Shelf Time: 24months Material: Plastic

A rapid, one step test for the qualitative detection of rotavirus and adenovirus in human feces.
 

For professional  CE certified

 

 

Applications: 

 

The Rotavirus and Adenovirus Combo Test Cassette (Feces) is a rapid chromatographic immunoassay for the qualitative detection of rotavirus and adenovirus in human feces specimens to aid in the diagnosis of rotavirus or adenovirus infection.

 

 

Description: 

 

Acute diarrhea disease in young children is a major cause of morbidity worldwide and is a leading cause of mortality in developing countries.1 Rotavirus is the most common agent responsible for acute gastroenteritis, mainly in young children.2 Its discovery in 1973 and its association with infantile gastroenteritis represented a very important advancement in the study of gastroenteritis not caused by acute bacterial infection. Rotavirus is transmitted by oral-fecal route with an incubation period of 1-3 days. Although specimen collections taken within the second and fifth day of the illness are ideal for antigen detection, the rotavirus may still be found while diarrhea continues. Rotaviral gastroenteritis may result in mortality for populations at risk such as infants, the elderly and immunocompromised patients.3 In temperate climates, rotavirus infections occur mainly in the winter months. Endemics as well as epidemics affecting some thousand people have been reported.4 With hospitalized children suffering from acute enteric disease up to 50% of the analyzed specimen were positive for rotavirus.5 The viruses replicate in the cell nucleus and tend to be host species specific producing a characteristic cytopathic effect (CPE). Because rotavirus is extremely difficult to culture, it is unusual to use isolation of the virus in diagnosing an infection. Instead, a variety of techniques have been developed to detect rotavirus in feces.

Research has shown that enteric adenoviruses, primarily Ad40 and Ad41, are a leading cause of diarrhea in many of these children, second only to the rotaviruses.6,7,8,9 These viral pathogens have been isolated throughout the world, and can cause diarrhea in children year round. Infections are most frequently seen in children less than two years of age, but have been found in patients of all ages. Further studies indicate that adenoviruses are associated with 4 - 15% of all hospitalized cases of viral gastroenteritis.5,6,7,8,9 Rapid and accurate diagnosis of gastroenteritis due to adenovirus is helpful in establishing the etiology of gastroenteritis and related patient management. Other diagnostic techniques such as electron microscopy (EM) and nucleic acid hybridization are expensive and labor-intensive. With the self-limiting nature of adenovirus infection, such expensive and labor-intensive tests may not be necessary.

The Rotavirus and Adenovirus Combo Test Cassette (Feces) is a rapid chromatographic immunoassay for the qualitative detection of rotavirus and adenovirus in human feces specimen, providing results in 10 minutes. The test utilizes antibody specific for rotavirus and adenovirus to selectively detect rotavirus and adenovirus from human feces specimens.

 

 

How to use? 

 

Allow the test cassette, specimen, and buffer to reach room temperature (15‑30°C) prior to testing.

  1. To collect fecal specimens:

Collect sufficient quantity of feces (1-2 ml or 1-2 g) in a clean, dry specimen collection container to obtain enough virus particles. Best results will be obtained if the assay is performed within 6 hours after collection. Specimen collected may be stored for 3 days at 2-8°C if not tested within 6 hours. For long-term storage, specimens should be kept below -20°C.

    2. To process fecal specimens:

· For Solid Specimens:

Unscrew the cap of the specimen collection tube, then randomly stab the specimen collection applicator into the fecal specimen in at least 3 different sites to collect approximately 50 mg of feces (equivalent to 1/4 of a pea). Do not scoop the fecal specimen.

· For Liquid Specimens:

Hold the dropper vertically, aspirate fecal specimens, and then transfer 2 drops of the liquid specimen (approximately 50 µL) into the specimen collection tube containing the extraction buffer.

Tighten the cap onto the specimen collection tube, then shake the specimen collection tube vigorously to mix the specimen and the extraction buffer. Leave the collection tube for reaction for 2 minutes.

3. Bring the pouch to room temperature before opening it. Remove the test cassette from the foil pouch and use it within one hour. Best results will be obtained if the test is performed immediately after opening the foil pouch.

4. Hold the specimen collection tube upright and open the cap on the tip. Invert the specimen collection tube and transfer 2 full drops of the extracted specimen (approximately 80μL) to the specimen well (S) of the test cassette, then start the timer. Avoid trapping air bubbles in the specimen well (S). See illustration below.

5. Read the results at 10 minutes after dispensing the specimen. Do not read results after 20 minutes.

Note: If the specimen does not migrate (presence of particles), centrifuge the extracted specimen contained in the extraction buffer vial. Collect 80 µL of supernatant, dispense into the specimen well (S). Start the timer and continue from step 5 onwards in the above instructions for use.High Quality And Convenient Professional Rotavirus / Adenovirus Combo Test Cassette

 

INTERPRETATION OF RESULTS
POSITIVE:
Rotavirus Positive: * A colored line appears in the control line region (C) and another colored line appears in the T1 line region.
Adenovirus Positive: * A colored line appears in the control line region (C) and another colored line appears in the T2 line region. Rotavirus and Adenovirus Positive: * A colored line appears in the control line region (C) and two other colored lines appear in T1 line region and T2 line region respectively.
*NOTE: The intensity of the color in the test line region (T1/T2) will vary depending on the concentration of rotavirus or adenovirus antigens present in the specimen. Therefore, any shade of color in the test line region (T1/T2) should be considered positive.
NEGATIVE: One colored line appears in the control line region (C). No line appears in the test line region (T1/T2).
INVALID: Control line (C) fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test with a new test cassette. If the problem persists, discontinue using the test kit immediately and contact your local distributor.

 
   
 
   

Contact Details
Hangzhou AllTest Biotech CO.,LTD

Tel:86-571-56267891

Fax:86-571-56267856

Contact Person: Mrs. Selina

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