Easy To Used Zika Ns1 Rapid Test Cassette In Whole Blood / Serum / Plasma

Easy To Used Zika Ns1 Rapid Test Cassette In Whole Blood / Serum / Plasma

A rapid test for a qualitative test for the detection of NS1 antigen of Zika virus in human whole blood, serum or plasma.

For professional in vitro diagnostic use only.

Applications:

The Zika NS1 Rapid Test Cassette (Whole Blood/Serum/Plasma) is a rapid chromatographic immunoassay for the qualitative detection of NS1 antigen of Zika virus in human whole blood, serum, or plasma as an aid in the diagnosis of Zika infections.

Description:

Zika virus (ZIKV) is a member of the virus family Flaviviridae.[1] It is spread by daytime-active Aedes mosquitoes, such as A. aegypti and A. albopictus.[1] Its name comes from the Zika Forest of Uganda, where the virus was first isolated in 1947.[2] Zika virus is related to the dengue, yellow fever, Japanese encephalitis, and West Nile viruses.[4] Since the 1950s, it has been known to occur within a narrow equatorial belt from Africa to Asia. From 2007 to 2016, the virus spread eastward, across the Pacific Ocean to the Americas, leading to the 2015–16 Zika virus epidemic.

The infection, known as Zika fever or Zika virus disease, often causes no or only mild symptoms, similar to a very mild form of dengue fever.[1] While there is no specific treatment, paracetamol (acetaminophen) and rest may help with the symptoms.[3] As of 2016, the illness cannot be prevented by medications or vaccines.[3] Zika can also spread from a pregnant woman to her fetus. This can result in microcephaly, severe brain malformations, and other birth defects.[4][5] Zika infections in adults may result rarely in Guillain–Barrésyndrome.[6]

The Zika NS1 Rapid Test Cassette (Whole Blood/Serum/Plasma) is a rapid test that utilizes a combination of Zika NS1 antibodies coated colored particles for the detection of Zika NS1 antigen in human whole blood, serum, or plasma.

How to use?

Allow the test, specimen, buffer and/or controls to reach room temperature (15-30°C) prior to testing.

   1.Bring the pouch to room temperature before opening it. Remove the test cassette from the sealed   pouch and use it within 1 hour.

   2.Place the cassette on a clean and level surface.

For Serum or Plasma specimen:

     Hold the dropper vertically and transfer 1 drop of serum or plasma (approximately 25 μL) to the specimen area, then add 1 drop (approx. 40μL) of buffer and start the timer. See illustration below.

For Venipuncture Whole Blood specimen:

· Hold the dropper vertically and transfer 2 drops of whole blood (approximately 50 μL) to the specimen area, then add 1 drop of buffer (approximately 40 μL), and start the timer. See illustration below.

For Fingerstick Whole Blood specimen:

• To use a capillary tube: Fill the capillary tube and transfer approximately 50 μL of fingerstick whole blood specimen to the specimen area of test cassette, then add 1 drop of buffer (approximately 40 μL) and start the timer. See illustration below.
• To use hanging drops: Allow 2 hanging drops of fingerstick whole blood specimen (approximately 50 μL) to fall into the specimen area of test cassette, then add 1 drop of buffer (approximately 40 μL) and start the timer. See illustration below.

• Wait for the colored line(s) to appear. Read results at 15 minutes. Do not interpret the result after 20 minutes.

INTERPRETATION OF RESULTS

(Please refer to the illustration above)

POSITIVE: * Two distinct colored lines appear. One color line should be in the control region (C) and another color line should be in the test region (T).

*NOTE: The intensity of the color in the test line region (T) will vary depending on the concentration of Zika NS1 antigen present in the specimen. Therefore, any shade of red in the test region should be considered positive.

NEGATIVE: One color line appears in the control region (C). No apparent red or pink line appears in the test region (T).

INVALID: Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test with a new test cassette. If the problem persists, discontinue using the test kit immediately and contact your local distributor.