500ng 1000ng Rapid Test to detection of Ethyl Glucuronide in human Whole Blood /Serum/Plasma with CE
The Ethyl Glucuronide (ETG) Rapid Test Cassette (Urine) is a rapid chromatographic immunoassay for the detection of Ethyl Glucuronide in human urine. The Ethyl Glucuronide detected by the test includes, but are not limited to, the metabolites of Ethanol.
This assay provides only a preliminary analytical test result. A more specific alternate chemical method must be used in order to obtain a confirmed analytical result. Gas chromatography/mass spectrometry (GC/MS) is the preferred confirmatory method. Clinical consideration and professional judgment should be applied to any drug of abuse test result, particularly when preliminary positive results are used.
Ethyl Glucuronide (ETG) is a metabolite of ethyl alcohol which is formed in the body byglucuronidation following exposure to ethanol, such as by drinking alcoholic beverages. Itisused as a biomarker to test for ethanol use and to monitor alcohol abstinence in situationswhere drinking is prohibited, such as in the military, in professional monitoring programs(health professionals, attorneys, airline pilots in recovery from addictions), in schools, inliver transplant clinics, or in recovering alcoholic patients. ETG can be measured in urine by Traditional laboratory methods (GC/MS or LC/MS) upto approximately 80 hours after ethanol is ingested (greatly dependent on the amount of alcoholic and non-alcoholic drinks). ETG is a more accurate indicator of therecent exposure to alcohol than measuring for the presence of ethanol itself.
The Ethyl Glucuronide Rapid Test Cassette (Urine) is a rapid urine screening test that can be performed without the use of an instrument. The test utilizes a monoclonal antibody to selectively detect elevated levels of ethyl glucuronide in human urine. The Ethyl Glucuronide Rapid Test Strip (Urine) yields a positive result when the Ethyl Glucuronide in urine exceeds 500 ng/mL.
How to use?
Allow the test, specimen, buffer and/or controls to reach room temperature (15-30°C) prior to testing.
For serum or plasma specimen:
1. Bring the pouch to room temperature (15-30℃) before opening it. Remove the cassette from the sealed pouch and use it within one hour.
2. Place the cassette on a clean and level surface. Hold the dropper vertically and transfer 1 full drop of serum or plasma (approximately 40μl), then add 2 drops of buffer (approximately 80μl) to the specimen well of the cassette, and then start the timer. Avoid trapping air bubbles in the specimen well. See illustration below.
3. Wait for the colored line(s) to appear. Read the result at 5 minutes. Do not interpret the result after 10 minutes.
1 Bring the pouch to room temperature before opening it. Remove the test cassette from the sealed pouch and use it as soon as possible.
2 Place the cassette on a clean and level surface.
For Venipuncture Whole blood specimen:
Hold the dropper vertically and transfer 1 drop of whole blood (approximately 40μl) to the specimen well, then add 2 drops of buffer (approximately 80μl), and start the timer. See illustration below.
For Fingerstick Whole blood specimen:
To use a capillary tube: Fill the capillary tube and transfer approximately 40μl of fingerstick Whole blood specimen to the specimen area of test cassette, then add 2 drops of buffer(approximately 80μl) and start the timer. See illustration below.
3 Wait for the colored line(s) to appear. Read results at 5 minutes. Do not interpret the result after 10 minutes.
INTERPRETATION OF RESULTS
(Please refer to the illustration above)
NEGATIVE:* Two lines appear. One colored line should be in the control line region (C), and another apparent colored line should be in the test line region (T). This negative result indicates that the Ketamine concentration is below the detectable cut-off level.
*NOTE: The shade of color in the test line region (T) may vary, but it should be considered negative whenever there is even a faint colored line.
POSITIVE: One colored line appears in the control line region (C). No line appears in the test line region (T). This positive result indicates that the Ketamine concentration exceeds the detectable cut-off level.
INVALID: Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test with a new test. If the problem persists, discontinue using the test kit immediately and contact your local distributor.