Insulin-like growth factor-binding protein 1 (IBP-1) known as placental protein 12 (PP12) is a protein
that in humans is encoded by the IGFBP1 gene
. IGF-binding proteins (IGFBPs) is believed to be important in the regulation of fetal and neonatal growth.
The Insulin-like growth factor-binding protein 1 (iGFBP-1) rapid test (vaginal secretion) is a visually interpreted, qualitative immunochromatographic test device for detection of iGFBP-1 in vaginal secretions during pregnancy, which is a major protein marker of the amniotic fluid in a vaginal sample. The test is intended for professional use to help diagnose the rupture of fetal membranes (ROM) in pregnant women.
Insulin-like growth factor-binding protein 1 (IBP-1) known as placental protein 12 (PP12) is a protein that in humans is encoded by the IGFBP1 gene. IGF-binding proteins (IGFBPs) is believed to be important in the regulation of fetal and neonatal growth. We previously reported that the profiles of IGFBPs in fetal cord serum (FCS) were dependent on the growth/metabolic status of the fetus. It can be detected in cervical secretions of pregnant women with preterm uterine contractions, and whether their presence predicts an increased risk of preterm delivery. The abundance of insulin-like growth factor binding protein-1 at the maternal-fetal interface in severely preeclamptic pregnancies suggests that the binding protein may participate in the pathogenesis of the shallow placental invasion observed in this disorder. Low circulating insulin-like growth factor-I and elevated insulin-like growth factor binding protein-1 levels may contribute to restricted placental and therefore fetal growth.
The iGFBP-1 Rapid Test Cassette (Vaginal secretion) is a rapid test that qualitatively detects the presence of iGFBP-1 in vaginal secretion specimen at the sensitivity of 25ng/ml.
How to use?
Bring test cassettes, specimens, buffer and/or controls to room temperature (15-30°C) before use.
1.Exact QR Code from the QR code card for specific information of the test. Use only QR code card provided inside the test kits.
2.Remove the test cassette from its sealed pouch, and place it on a clean, level surface. Label the test with patient or control identification. To obtain a best result, the assay should be performed within one hour.
3.Insert the swab into the dilute tube, rotate for 20 times. Then press the swab against the side of the tube and squeeze the bottom of the tube as the swab is withdrawn. Discard the swab.
4.Fit the cap onto the tube. Remove the top part of the cap. Place the test cassette on a clean and level surface. Add 3 full drops of solution (approx. 100 µL) to the specimen well (S) and then start the timer.
5.Wait for the colored band to appear. The result should be read with the AllTestTM LF Reader at 5 minutes.
6.Do not interpret test results visually.
Note: For the AllTestTM LF Reader installation, start up and complete instructions refer to the AllTestTM LF Reader User Manual. Operator must consult the AllTestTM LF Reader User Manual prior to use and become familiar with the processes and quality control procedures.
INTERPRETATION OF RESULTS
Results read by Alltest LF reader
The result of positive or negative for binding protein 1 (IBP-1) is determined by the Alltest LF reader.
Result could be exported to excel.