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Fast Reading Sensitive Accurate Calprotectin and FOB Rapid Test Cassette One Step Disposable for Diagnosis with CE

Product Details:

Place of Origin:China
Brand Name:AllTest
Certification:CE, ISO13485
Model Number:Cassette (TCFC-625)

Payment & Shipping Terms:

Minimum Order Quantity:N/A
Price:Negotiation
Packaging Details:10 cassette/box
Delivery Time:2-4 Weeks
Payment Terms:Ex works
Supply Ability:10 M tests/month
Detailed Product Description
Format: Cassette Specimen: Feces
Accuracy: 99.% Certificate: ISO13485,CE
Storage: 2-30℃ Shelf Time: 24 Months
Cut-off: See Insert

 
Sensitive Accurate Calprotectin and FOB Rapid Test Cassette One Step Disposable for Diagnosis with fast reading

 

Applications:

The Calprotectin and FOB Combo Rapid Test Cassette (Feces) is a rapid chromatographic immunoassay for the qualitative detection of calprotectin and/or Human Occult Blood in feces.
 
Description:  
 
Calprotectin is a 24 kDa dimer of calcium binding proteins S100A8 and S100A9. The complex accounts for up to 60% of the soluble protein content of the neutrophil cytosol. Calprotectin becomes available in the intestinal lumen via leukocyte shedding, active secretion, cell disturbance, and cell death. 3This results in elevated faecal calprotectin levels, which can be detected in the stool. Elevated faecal calprotectin levels therefore indicate migration of neutrophils into the intestinal mucosa, which occurs during intestinal inflammation. Faecal calprotectin has been used to detect intestinal inflammation, and can serve as a marker for inflammatory bowel diseases. Calprotectin is useful as a marker, as it is resistant to enzymatic degradation, and can be easily measured in faeces.
Many diseases can cause hidden blood in the feces. This is also known as Fecal Occult Blood (FOB), Human Occult Blood, or Human Hemoglobin. In the early stages, gastrointestinal problems such as colon cancer, ulcers, polyps, colitis, diverticulitis, and fissures may not show any visible symptoms, only occult blood. Traditional guaiac-based methods lack sensitivity and specificity, and also have diet restrictions prior to testing.
 
How to use? 

 

Allow the test, specimen, buffer and/or controls to reach room temperature (15-30°C) prior to testing.
1.To collect fecal specimens:
Collect sufficient quantity of feces (1-2mL or 1-2g) in a clean, dry specimen collection container to obtain maximum antigens (if present). Best results will be obtained if the assay is performed within 6 hours after collection. Specimen collected may be stored for 3 days at 2-8℃ if not tested within 6 hours. For long term storage, specimens should be kept below -20℃.
2.To process fecal specimens:
• For Solid Specimens:
Unscrew the cap of the specimen collection tube,then randomly stab the specimen collection applicator into the fecal specimen in at least 3 different sites to collect approximately 50 mg of feces (equivalent to 1/4 of a pea). Do not scoop the fecal specimen.
• For Liquid Specimens:
Hold the dropper vertically, aspirate fecal specimens, and then transfer 2 drops (approximately 80μL) into the specimen collection tube containing the extraction buffer.
3.Tighten the cap onto the specimen collection tube, then shake the specimen collection tube vigorously to mix the specimen and the extraction buffer. Leave the tube alone for 2 minutes.
4.Bring the pouch to room temperature before opening it. Remove the test cassette from the foil pouch and use it within one hour. Best results will be obtained if the test is performed immediately after opening the foil pouch.
5.Hold the specimen collection tube upright and open the cap onto the specimen collection tube. Invert the specimen collection tube and transfer 2 full drops of the extracted specimen (approximately 80μL) to the each specimen well (S) of the test cassette, then start the timer. Avoid trapping air bubbles in the specimen well (S). See illustration below.
6.Read results at 5 minutes after dispensing the specimen. Do not read results after 10 minutes.
Note: If the specimen does not migrate (presence of particles), centrifuge the extracted specimens contained in the extraction buffer vial. Collect 80ul of supernatant, dispense into the specimen well (S) of a new test cassette and start afresh following the instructions mentioned above.

 

Fast Reading Sensitive Accurate Calprotectin and FOB Rapid Test Cassette One Step Disposable for Diagnosis with CE

 

 

 

 

 

INTERPRETATION OF RESULTS
(Please refer to the illustration)
POSITIVE:* Two colored lines appear. One colored line should be in the  control line region (C) and another apparent colored line should be in the  test line region (T).
*NOTE: The intensity of the color in the test line region (T) will vary depending on the concentration of Calprotectin and/or FOB present in the specimen. Therefore, any shade of color in the test line region (T) should be considered positive.
NEGATIVE: One colored line appears in the control line region (C). No line appears in the test line region (T).
INVALID: Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test with a new test. If the problem persists, discontinue using the test kit immediately and contact your local distributor.

 

 

 

Cat. No. Product Description Specimen Format Kit Size Cut-Off Status
TCFC-625 Calprotectin and FOB Combo Rapid Test Cassette Feces Cassette 10 T See Insert CE

Contact Details
Hangzhou AllTest Biotech CO.,LTD

Tel:86-571-56267891

Fax:86-571-56267856

Contact Person: Mrs. Selina

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