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                   Hangzhou AllTest Biotech CO.,LTD 

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Haptoglobin Hemoglobin Combo Rapid Test Kits Rapid Std Testing Kits

Product Details:

Place of Origin:CHINA
Brand Name:AllTest
Certification:CE
Model Number:cassette

Payment & Shipping Terms:

Minimum Order Quantity:500
Packaging Details:25 tests per kit
Supply Ability:100 Million a year
Detailed Product Description
Format: Cassette Specimen: Feces
Kit Size: 25T/Kit Cut-Off: 50ng/mL
Color: Green Certificate: CE

 

A rapid, one step test for the qualitative detection of Hemoglobin or Haptoglobin-Hemoglobin complex CE certified

 

 

Applications: 

 

The Hb+Hb/Hp Combo Rapid Test Cassette (Feces) is a rapid chromatographic immunoassay for the qualitative detection of hemoglobin or haptoglobin-hemoglobin complex in feces.

 

 

Description: 

 

The Hb+Hb/Hp Combo Rapid Test is a sandwich immunoassay. It is a panel test consisting of 2 individual tests, a hemoglobin (Hb) test and a hemoglobin-haptoglobin (Hb-Hp) complex test to selectively identify human Hb and Hb-Hp complexes in test samples. Each test contains a nitrocellulose membrane strip with an immobilized mouse anti- Hb -antibody on the test zone (T). The Hb test has a pad containing poly anti-Hb colloidal gold and mouse IgE colloidal gold. The Hb-Hp complex test has a pad containing poly anti-Hp colloidal gold and mouse IgE colloidal gold. A goat-anti-mouse antibody is immobilized in the control reaction zone (C) on the nitrocellulose membrane. 90 to 120μl specimen (buffer mixed) sample (containing the respective analytes, Hb and Hb/Hp complexes) is added onto the respective specimen pad. The analytes (i.e. Hb and/or Hb-Hp complex) in the collected specimen couple with the mouse anti-Hb-antibody or anti-Hp-antibody of the gold conjugate pad thus forming antibody – antigen – colloidal gold complexes. While the liquid is moving along the membrane it transports these complexes by capillary action. When the antibody – antigen – colloidal gold complexes are transported across the membrane and reach the respective immobilized mouse anti- Hb-antibody on the membrane, they are trapped and will form a sandwich complex consisting of: immobilized antibody – antigen – antibody – colloidal gold for Hb and/or Hb/Hp complex respectively. Only when the applied specimen sample contains a certain concentration of Hb or Hb/Hp complex, the formation of this sandwich complex will result in a visible purple color band in the respective test zones (T) of the membrane.

The colored C-line will always appear if the test has been performed correctly. The test sensitivity for hb is 50ng/ml and hb/hp is 50ng/ml.

 

 

How to use? 

 

Allow the test, specimen, buffer and/or controls to reach room temperature (15-30°C) prior to testing.

1.  To collect fecal specimens:

Collect feces in a clean, dry specimen collection container. Best results will be obtained if the assay is performed within 6 hours after collection. Specimen collected may be stored for 3 days at 2-8°C if not tested within 6 hours.

2.  To process fecal specimens:

Unscrew the cap of the specimen collection tube,then randomly stab the specimen collection applicator into the fecal specimen in at least 3 different sites. Do not scoop the fecal specimen.

Screw on and tighten the cap onto the specimen collection tube, then shake the specimen collection tube vigorously to mix the specimen and the extraction buffer. Specimens prepared in the specimen collection tube may be stored for 6 months at -20°C if not tested within 1 hour after preparation.

3.  Bring the pouch to room temperature before opening it. Remove the test cassette from the foil pouch and use it as soon as possible. Best results will be obtained if the test is performed immediately after opening the foil pouch.

4.  Hold the specimen collection tube upright and open the cap onto the specimen collection tube. Invert the specimen collection tube and transfer 3 full drops of the extracted specimen (approximately 120μL) to the specimen well (S) of the test cassette, then start the timer. Avoid trapping air bubbles in the specimen well (S). See illustration below.

5.  Read results at 5 minutes. Do not read results after 10 minutes.

Haptoglobin Hemoglobin Combo Rapid Test Kits Rapid Std Testing Kits

 

 
   

INTERPRETATION OF RESULTS

 

(Please refer to the illustration above)

 

POSITIVE:* Two lines appear. One colored line should be in the control line region (C) and another apparent colored line should be in the test line region (T).

 

*NOTE: The intensity of the color in the test line region (T) will vary depending on the concentration of hemoglobin or haptoglobin-hemoglobin complex present in the specimen. Therefore, any shade of color in the test line region (T) should be considered positive.

 

NEGATIVE: One colored line appears in the control line region (C). No line appears in the test line region (T).

 

INVALID: Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test with a new test. If the problem persists, discontinue using the test kit immediately and contact your local distributor.

 

 

QUALITY CONTROL

     

Internal procedural controls are included in the test. A colored line appearing in the control region (C) is an internal valid procedural control. It confirms sufficient specimen volume and correct procedural technique.

Control standards are not supplied with this kit; however, it is recommended that positive and negative controls be tested as a good laboratory practice to confirm the test procedure and to verify proper test performance.

Contact Details
Hangzhou AllTest Biotech CO.,LTD

Tel:86-571-56267891

Fax:86-571-56267856

Contact Person: Mrs. Selina

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