AllTest Filariasis Rapid Test Kits Blood & Serum & plasma IFIL-402

A rapid test for the qualitative detection of antibodies to Filarial parasites (W. bancrofti and B. malayi) in human whole blood, serum or plasma CE certified

Applications:

The Filariasis Rapid Test Cassette (Whole Blood/Serum/Plasma) is a rapid chromatographic immunoassay for the qualitative detection of antibodies to Filarial parasites (W. bancrofti and B. ma layi) in whole blood, serum, or plasma to aid in the diagnosis of Filariasis.

Description:

The lymphatic filariasis known as Elephantiasis, mainly caused by W. bancrofti and B. malayi, affects about 120 million people over 80 countries^[1],[2]. The disease is transmitted to humans by the bites of infected mosquitoes within which the microflariae sucked from an infected human subject develops into third-stage larvae. Generally, repeated and prolonged exposure to infected larvae is required for establishment of human infection.

The definitive parasitologic diagnosis is the demonstration of microflariae in blood samples³. However, this gold standard test is restricted by the requirement for nocturnal blood collection and lack of adequate sensitivity. Detection of circulating antigens is commercially available. Its usefulness is limited for W. bancrofti⁴. In addition, microfilaremia and antigenemia develop from months to years after exposure.

Antibody detection provides an early means to detect filarial parasite infection. Presence of IgM to the parasite antigens suggest current infection, whereas, IgG corresponds to late stage of infection or past infection⁵. Furthermore, identification of conserved antigens allows ‘pan-filaria’ test to be applicable. Utilization of recombinant proteins eliminates cross-reaction with individuals having other parasitic diseases⁶. The Filariasis Rapid Test uses conserved recombinant antigens to simultaneously detect antibodies to the W. bancrofti and B. malayi parasites without the restriction on specimen collection.

How to use?

Allow the test, specimen, buffer and/or controls to reach room temperature (15-30°C) prior to testing.

• Bring the pouch to room temperature before opening it. Remove the test cassette from the sealed pouch and use it as soon as possible. Best results will be obtained if the assay is performed within one hour.
• Place the cassette on a clean and level surface.

For Serum or Plasma specimen: Hold the dropper vertically and transfer 1 drop of serum or plasma (approximately 40mL) to the specimen area,then add 2 drops of buffer (approximately 80 mL),and start the timer, see illustration below.

For Venipuncture Whole Blood specimen: Hold the dropper vertically and transfer 1 drop of whole blood (approximately 40mL) to the specimen area, then add 2drops of buffer (approximately 80mL), and start the timer. See illustration below.

For Fingerstick Whole Bloodspecimen:

• To use a capillary tube: Fill the capillary tube and transfer approximately 40mL of fingerstick whole blood specimen to the specimen area of test cassette, then add 2 drops of buffer (approximately 80 mL) and start the timer. See illustration below.
• To use hanging drops: Allow 1 hanging drop of fingerstick whole blood specimen (approximately 40mL) to fall into the specimen area of test cassette, then add 2 drops of buffer (approximately 80 mL) and start the timer. See illustration below.

• Wait for the colored line(s) to appear. Read the result at 15 minutes, do not interpret the result after 20 minutes.

INTERPRETATION OF RESULTS

(Please refer to the illustration above)

POSITIVE:* Two distinct colored lines appear. One colored line should be in the control region (C) and another colored line should be in the test region (T).

*NOTE: The intensity of the color in the test line region (T) will vary depending on the concentration of Filariasis antibody present in the specimen. Therefore, any shade of color in the test region (T) should be considered positive.

NEGATIVE: One colored line appears in the control region (C). No apparent colored line appears in the test region (T).

INVALID: Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test with a new test cassette. If the problem persists, discontinue using the test kit immediately and contact your local distributor.