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|Kit Size:||10T/Kit||Cut-Off:||30 Ng/mL|
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Ferritin Semi-Quantitative Rapid Test Cassette in Whole Blood / Serum / Plasma with High Quilified
The Ferritin Rapid Test Cassette is a rapid chromatographic immunoassay for the qualitative detection of human ferritin in human serum or plasma and whole blood at a cut-off concentration of 30ng/ml.
Ferritin is a universal intracellular protein that stores iron and releases it in a controlled fashion. Plasma ferritin is an indirect marker of the total amount of iron stored in the body; hence serum ferritin is used as a diagnostic test for iron deficiency anemia.
A normal ferritin blood level, referred to as the reference interval is determined by many testing laboratories. In most tissues, ferritin is a major iron storage protein. Human ferritin has a molecular weight of approximately 450,000 Daltons, and consists of a protein shell around an iron core; each molecule of ferritin may contain as many as 4,000 iron atoms. Under normal conditions, this may represent 25% of the total iron found in the body. In addition, ferritin can be found in several isomers. If the ferritin level is low, there is a risk for lack of iron, which could lead to anemia. Low ferritin may also indicate hypothyroidism, vitamin C deficiency or celiac disease. Low serum ferritin levels are seen in some patients with restless legs syndrome, not necessarily related to anemia, but perhaps due to low iron stores short of anemia.
How to use?
Allow the test, specimen, buffer and/or controls to reach room temperature (15-30°C) prior to testing
For serum or plasma specimen:
1. Bring the pouch to room temperature (15-30℃) before opening it. Remove the cassette from the sealed pouch and use it within one hour.
2. Place the cassette on a clean and level surface. Hold the dropper vertically and transfer 1 full drop of serum or plasma (approximately 25ul) to the specimen well of the cassette, then add 1 drop of buffer (approximately 40ml) and start the timer. Avoid trapping air bubbles in the specimen well. See illustration below.
3. Wait for the colored line(s) to appear. Read the result at 5 minutes when testing a serum or plasma specimen.
For whole blood specimen:
1. Bring the pouch to room temperature before opening it. Remove the test cassette from the sealed pouch and use it as soon as possible.
2. Place the cassette on a clean and level surface.
For Venipuncture Whole Blood specimen:
Hold the dropper vertically and transfer 2 drops of whole blood (approximately 50 ul) to the specimen well, then add 1 drop of buffer (approximately 40 ul), and start the timer. See illustration below.
For Fingerstick Whole Blood specimen:
To use a capillary tube: Fill the capillary tube and transfer approximately 50 ul of fingerstick whole blood specimen to the specimen well of test cassette, then add 1 drop of buffer (approximately 40 ul) and start the timer. See illustration below.
3. Wait for the colored line(s) to appear. Read result at 5 minutes. Do not interpret the result after 10 minutes.
INTERPRETATION OF RESULTS
(Please refer to the illustration)
≥30ng/ml: Two lines appear. The line in test line region (T) appears. It depicts no iron deficiency anemia, unless Ferritin levels are raised due to some other reasons.
＜30ng/ml: One line appears. Only control line appears(C). There may be a risk of anemia.
INVALID: Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test with a new test. If the problem persists, discontinue using the test kit immediately and contact your local distributor.
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