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Rotavirus Convenient Infectious Disease Testing Cassette One Step Rapid Test

Product Details:

Place of Origin:CHINA
Brand Name:ALLTEST
Certification:CE
Model Number:Cassette(IRO-602)

Payment & Shipping Terms:

Packaging Details:10T
Detailed Product Description
Color: Blue Format: Cassette
Storage: 2-30°C Shelf Time: 24months
Certificate: CE Cut-off: See Insert

 
A rapid, one step test for the qualitative detection of Adenovirus in human feces. For professional in vitro diagnostic use only.
 
Application:
 

The Rotavirus Rapid Test Cassette (Feces) is a rapid chromatographic immunoassay for the qualitative detection of rotavirus in human feces specimens to aid in the diagnosis of rotavirus infection.The Adenovirus Rapid Test Cassette (Feces) is a rapid chromatographic immunoassay for the qualitative detection of Adenovirus in human feces specimens to aid in the diagnosis of adenovirus infection.

 
Description:
 

Rotavirus is the most common agent responsible for acute gastroenteritis, mainly in young children. Its discovery in 1973 and its association with infantile gastro-enteritis represented a very important advancement in the study of gastro-enteritis not caused by acute bacterial infection. Rotavirus is transmitted by oro-faecal route with an incubation period of 1-3 days.
Although specimen collections taken within the second and fifth day of the illness are ideal for antigen detection, the rotavirus may still be found while diarrhoea continues. Rotaviral gastroenteritis may result in mortality for populations at risk such as infants, the elderly, and immunocompromised patients. In temperate climates, rotavirus infections occur mainly in the winter months. Endemics as well as epidemics affecting some thousand people have been reported. With hospitalised children suffering from acute entric disease up to 50% of the analysed specimen were positive for rotavirus. The viruses replicate in the cell nucleus and tend to be host species specific producing a characteristic cytopathic effect (CPE). Because rotavirus is extremely difficult to culture, it is unusual to use isolation of the virus in diagnosing an infection. Instead, a variety of techniques have been developed to detect rotavirus in feces.
The Rotavirus Rapid Test Cassette (Feces) is a rapid chromatographic immunoassay for the qualitative detection of rotavirus in human feces specimen, providing results in 10 minutes. The test utilizes antibody specific for rotavirus to selectively detect rotavirus from human feces specimens.

 

How to use?

 

Allow the test, specimen, buffer, and/or controls to reach room temperature (15-30°C) prior to testing.
1. To collect fecal specimens:
Collect sufficient quantity of feces (1-2 mL or 1-2 g) in a clean, dry specimen collection container to obtain enough virus particles. Best results will be obtained if the assay is performed within 6 hours after collection. Specimen collected may be stored for 3 days at 2- 8°C if not tested within 6 hours. For long term storage, specimens should be kept below - 20°C.
2. To process fecal specimens:
For Solid Specimens:
Unscrew the cap of the specimen collection tube, then randomly stab the specimen collection applicator into the fecal specimen in at least 3 different sites to collect approximately 50 mg of feces (equivalent to 1/4 of a pea). Do not scoop the fecal specimen.
For Liquid Specimens:
Hold the dropper vertically, aspirate fecal specimens, and then transfer 2 drops of the liquid specimen (approximately 50 µL) into the specimen collection tube containing the extraction buffer.
Tighten the cap onto the specimen collection tube, then shake the specimen collection tube vigorously to mix the specimen and the extraction buffer. Leave the collection tube for reaction for 2 minutes.
3. Bring the pouch to room temperature before opening it. Remove the test cassette from the foil pouch and use it as soon as possible. Best results will be obtained if the test is performed immediately after opening the foil pouch.
4. Hold the specimen collection tube upright and unscrew the tip of the specimen collection tube. Invert the specimen collection tube and transfer 2 full drops of the extracted specimen (approximately 80 mL) to the specimen well (S) of the test cassette, then start the timer. Avoid trapping air bubbles in the specimen well (S). See illustration below.
5. Read the results at 10 minutes after dispensing the specimen. Do not read results after 20 minutes.
Note: If the specimen does not migrate (presence of particles), centrifuge the diluted sample in the extraction buffer vial. Collect 80 µL of supernatant, dispense into the specimen well (S). Start the timer and continue from step 5 onwards in the above instructions for use.

Rotavirus Convenient Infectious Disease Testing Cassette One Step Rapid Test

 

INTERPRETATION OF RESULTS 


(Please refer to the illustration above)


POSITIVE:* Two distinct colored lines appear. One colored line should be in the control line region (C) and another apparent colored line should be in the test line region (T).
*NOTE: The intensity of the color in the test line region (T) will vary depending on the concentration of rotavirus antigen present in the specimen. Therefore, any shade of color in the test line region (T) should be considered positive.
NEGATIVE: One colored line appears in the control line region (C). No line appears in the test line region (T).
INVALID: Control line (C) fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test with a new test cassette. If the problem persists, discontinue using the test kit immediately and contact your local distributor.

 

Cat. No. Product Description Specimen Format Kit Size Cut-Off Status
IRO-602 Rotavirus Rapid Test Cassette Feces Cassette 25 T See Insert CE
 

Contact Details
Hangzhou AllTest Biotech CO.,LTD

Tel:86-571-56267891

Fax:86-571-56267856

Contact Person: Mrs. Selina

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