Dengue IgG / IgM Ab Rapid Test Cassette One Step Diagnosis Blood Testing tropical infectious disease testing igg test

 
Dengue IgG / IgM Ab Rapid Test Kit One Step Diagnosis Blood Testing
 
 
Applications:
 
The Dengue IgG/IgM Ab Rapid Test Cassette (Whole Blood/Serum/Plasma) is a qualitative membrane-based immunoassay for the detection of Dengue antibodies in whole blood, serum, or plasma.

 
Description:  
 
The Dengue IgG/IgM Ab Rapid Test Cassette (Whole Blood / Serum/ Plasma) is a qualitative membrane-based immunoassay for the detection of Dengue antibodies in whole blood, serum, or plasma. This test consists of two components, an IgG component and an IgM component. In the IgG component, anti-human IgG is coated in IgG test line region. During testing, the specimen reacts with Dengue antigen coated particles in the test cassette. The mixture then migrates upward on the membrane chromatographically by capillary action and reacts with the anti-human IgG in IgG test line region. If the specimen contains IgG antibodies to Dengue, a colored line will appear in IgG test line region. In the IgM component, anti-human IgM is coated in IgM test line region. During testing, the specimen reacts with antihuman IgM. Dengue IgM antibodies, if present in the  specimen, reacts with the antihuman IgM and the Dengue antigen-coated  particles in the test cassette, and this complex is captured by the anti-human IgM, forming a colored line in IgM test line region.
Therefore, if the specimen contains Dengue IgG antibodies, a colored line will appear in t IgG test line region. If the specimen contains Dengue IgM antibodies, a colored line will appear in IgM test line region. If the specimen does not contain Dengue antibodies, no colored line will appear in either of the test line regions, indicating a negative result.
 
 
How to use? 
 
Allow the test cassette, specimen, buffer, and/or controls to reach room temperature (15-30°C) prior to testing.
1. Bring the pouch to room temperature before opening. Remove the test cassette from the sealed pouch and use it within one hour.
2. Place the test cassette on a clean and level surface.
· For Serum or Plasma Specimens:
To use a dropper: Hold the dropper vertically, draw the specimen up to the Fill Line (approximately 5 μl), and transfer the specimen to the specimen well of the test cassette, then add 1 drop of buffer (approximately 40ul) and start the timer. Avoid trapping air bubbles in the specimen well.
To use a micropipette: Pipette and dispense 5 µl of specimen to the specimen well of the test
cassette, then add 1 drop of buffer (approximately 40 µl) and start the timer.
· For Whole Blood (Venipuncture/Fingerstick) Specimens:
To use a dropper: Hold the dropper vertically, draw the specimen about 1cm above the Fill Line,
and transfer 1 drop of whole blood (approximately 10 µl) to the specimen well of the test cassette, then add 1 drop of buffer (approximately 40 ul) and start the timer.
To use a micropipette: Pipette and dispense 10 µl of whole blood to the specimen well of the test
cassette, then add 1 drop of buffer (approximately 40 µl) and start the timer.
3. Wait for the colored line(s) to appear. The test result should be read at 10 minutes. Do not interpret the result after 20 minutes.

 
 
 
INTERPRETATION OF RESULTS 
(Please refer to the illustration above)
IgG and IgM POSITIVE:* Three lines appear. One colored line should be in the control line region (C), and two colored lines should appear in IgG test line region and IgM test line region. The color intensities of the lines do not have to match. The result is positive for IgG & IgM antibodies indicated end stage of primary Dengue infection and early stage of secondary Dengue infection .
IgG POSITIVE:* Two lines appear.One colored line should be in the control line region (C), and a colored line appears in IgG test line region. The result is positive for Dengue virus specific-IgG and is probably indicative of secondary Dengue infection.
IgM POSITIVE:* Two lines appear.One colored line should be in the control line region (C), and a colored line appears in IgM test line region. The result is positive for Dengue virus specific-IgM antibodies and is indicative of primary Dengue infection.
*NOTE: The intensity of the color in the IgG and/or IgM test line region(s) will vary depending on the concentration of Dengue antibodies in the specimen. Therefore, any shade of color in the IgG and/or
IgM test line region(s) should be considered positive.
NEGATIVE: One colored line should be in the control line region (C). No line appears in IgG and IgM test line region(s).
INVALID: Control line fails to appear. Insufficient buffer volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the procedure with a new test cassette. If the problem persists, discontinue using the test kit immediately and contact your local distributor.
 

 
 
 
 
What to Know About Dengue Fever as Cases Spread to New Places
 

Dengue fever, a viral illness transmitted by Aedes mosquitoes, is experiencing a surge in cases worldwide. This increase is observed not only in regions that have long struggled with the disease but also in areas where its presence was previously unheard of, such as France, Italy, and Chad in central Africa. Recently, the United States reported its first case of locally transmitted dengue.

Factors contributing to this global rise in dengue cases include increased international travel, urbanization, and climate change, which create favorable conditions for mosquito breeding and viral transmission. The recent emergence of locally transmitted dengue in the United States is a concerning development, highlighting the expanding geographical range of the disease.

Dengue, also known as "breakbone fever," can cause severe joint pain and is transmitted by Aedes mosquitoes. While only a quarter of dengue cases exhibit symptoms, some infections may result in mild flu-like illness, while others can lead to more severe symptoms such as intense headaches, vomiting, high fever, and joint pain. It may take several weeks for patients to fully recover. Approximately 5 percent of infected individuals progress to severe dengue, which causes organ failure and shock in some patients.

Unfortunately, there is currently no specific treatment available for dengue infection. Patients are typically provided with symptom management, such as pain medication, to alleviate discomfort. However, pharmaceutical companies are actively conducting clinical trials to explore potential antiviral drugs for treating dengue. Alongside medical interventions, public health initiatives stress the importance of mosquito control measures. These include eliminating mosquito breeding sites and using insect repellents to reduce the transmission of dengue and minimize its impact on affected communities.