products details

Home Products

Flourescence Immunoassay Test cassette for Cardiac Troponin T Detection in Whole Blood/Serum/Plasma

Brand Name:	ALLTEST Novatrend Plus
Model Number:	FI-CTNT-402
MOQ:	N/A
Price:	negotiation
Payment Terms:	Ex works

Detail Information

Product Description

Detail Information

Time To Result:

15 Minutes

Sample:

Whole Blood/Serum/Plasma/Tissue

Sensitivity:

High

Pack:

10T/25T

Type:

Diagnostic Test Kits

Storage:

2-30℃

Test Type:

Flourescence Immunoassay

Shelf Time:

24months

Test Range:

0.2-40 Ng/mL

Highlight:

mouse monoclonal antibodies

hybridoma Monoclonal antibodies

Product Description

The Cardiac Troponin T Test Cassette (Whole Blood/Serum/Plasma) is intended for in vitro

quantitative determination of human cardiac Troponin T in whole blood, serum or plasma as an

aid in the diagnosis of Myocardial Infarction (MI).

【SUMMARY】

Cardiac Troponin T(cTnT) is a structurally bound protein found in striated muscle cells with a

molecular weight of 37kD.1 Troponin T is part of a three subunit complex comprising of Troponin

I and Troponin C. Along with tropomyosin, this structural complex forms the main component that

regulates the calcium sensitive ATPase activity of actomyosin in striated skeletal and cardiac

muscle.2 After acute myocardial infarction (AMI), serum cTnT levels are elevated 2 to 8 hours

after onset, peak in 12-24 hours and can persist for up to 14 days.3 Cardiac Troponin T(cTnT) as

currently recognized as the most valuable diagnostic index for myocardial injury, has shown

broad application prospects and replaced creatine phosphate kinase MB isoenzyme (CK-MB) as

the "gold standard" for judging myocardial injury, especially for diagnosing acute myocardial

infarction. It plays an important role in the diagnosis of heart failure, unstable angina pectoris,

myocarditis, drug-induced myocardial injury, cardiac injury monitoring in thoracic surgery,

various critical diseases and multiple organ failure.4

The Cardiac Troponin T Test Cassette (Whole Blood/Serum/Plasma) is a simple test that utilizes

a combination of anti-cTnT antibody coated particles and capture reagent to detect cTnT in

whole blood, serum or plasma.

【PRINCIPLE】

The Cardiac Troponin T Test Cassette (Whole Blood/Serum/Plasma) detects cardiac Troponin T

(cTnT) based on Fluorescence Immunoassay. The sample moves through the strip from sample

pad to absorbent pad. If the specimen contains cTnT, it attaches to the fluorescent

microspheres-conjugated anti-cTnT antibodies. Then the complex will be captured by the

capture antibodies coated on the nitrocellulose membrane (Test line). The concentration of cTnT

in the sample correlates with the fluorescence signal intensity captured on the T line. According

to the fluorescence intensity of the test and product standard curve, the concentration of cTnT in

the sample can be calculated by Analyzer to show cTnT concentration in specimen.

【REAGENTS】

The test kit includes anti-cTnT antibody coated fluorophores and anti-cTnT antibody coated on

the membrane.

【PRECAUTIONS】

1. For professional in vitro diagnostic use only.

2. Do not use after the expiration date indicated on the package. Do not use the test if the foil

pouch is damaged. Do not reuse.

3.Avoid cross-contamination of specimens by using a new specimen collection container for

each specimen obtained.

4. Do not eat, drink or smoke in the area where the specimens and tests are handled. Handle all

specimens as if they contain infectious agents. Observe established precautions against

microbiological hazards throughout the procedure and follow standard procedures for proper

disposal of specimens. Wear protective clothing such as laboratory coats, disposable gloves

and eye protection when specimens are assayed.

5. Do not interchange or mix reagents from different lots.

6. Humidity and temperature can adversely affect results.

7. Used testing materials should be discarded in accordance with local regulations.

8. Read the entire procedure carefully prior to any testing.

9. The cTnT Test Cassette should only be used with the Analyzer by approved

medical professionals.

【STORAGE AND STABILITY】

1. The test should be stored at 4-30°C until the expiry date printed on the sealed pouch.

2. The test must remain in the sealed pouch until use.

3. Do not freeze.

4. Care should be taken to protect the components of the kit from contamination.

5. Do not use if there is evidence of microbial contamination or precipitation. Biological

contamination of dispensing equipment, containers or reagents can lead to false results.

【SPECIMEN COLLECTION AND PREPARATION】

Preparation

1. Before performing the test, please make sure that all components are brought to room

temperature (15-30°C). Cold buffer solution or moisture condensation on the membrane can

lead to invalid test results.

2. Take a tube with buffer solution out of the kit. Document patients name or ID on it.

Sample Handling

1. Collect the specimen according to standard procedures.

2. Do not leave specimens at room temperature for prolonged periods. Serum and plasma

specimens may be stored at 2-8°C for up to 1 day, for long term storage, specimens should be

kept below -20°C. Whole blood collected by venipuncture should be stored at 2-8°C if the test

is to be used within 1 day of collection. Do not freeze whole blood specimens. Whole blood

collected by finger stick should be tested immediately.

3. Bring specimens to room temperature prior to testing. Frozen specimens must be completely

thawed and mixed well prior to testing. Avoid repeated freezing and thawing of specimens.

4. EDTA K2, Heparin sodium, Citrate sodium and Oxalate potassium can be used as the

anticoagulant tube for collecting the blood specimen.

Sample Dilution

1. The specimen (75μL of serum/plasma/ whole blood) can be added directly with the micro

pipette into the buffer.

2. Close the tube and shake the sample by hand for approximately 10 seconds so sample and

dilution buffer mix well.

3. Let the diluted sample homogenize for approximately 1 minute.

4. It is best to place the diluted sample on an ice pack and leave the sample at room temperature

for no more than 2 hours.

【MATERIALS】

Materials Provided

• Test Cassettes

• Specimen Collection Tubes with Buffer

• ID Card

• Package Insert

Materials Required But Not Provided

• Timer

• Centrifuge

• Fluorescence Immunoassay Analyzer

• Pipette

【DIRECTIONS FOR USE】

Refer to Fluorescence Immunoassay Analyzer Operation Manual for the complete

instructions on use of the Test. The test should be conducted at room temperature.

Allow the test, specimen, buffer and/or controls to reach room temperature (15-30 °C)

prior to testing.

1. Turn on the Analyzer power. Then according to the need, select “standard test” or “Quick

test” mode.

2. Take out the ID card and insert it into the Analyzer port.

3. Serum/plasma: Transfer 75μL of serum/plasma into the buffer tube; mix the specimen and

the buffer well.

Whole blood: Transfer 75μL of whole blood into the buffer tube with pipette; mix the

specimen and the buffer well.

4. Add diluted specimen with a Pipette: Pipette 75μL of diluted specimen into the sample

well. Start the timer at the same time.

5. There are two test modes for Fluorescence Immunoassay Analyzer, Standard

Test mode and Quick Test mode. Please refer to the user manual of

Fluorescence Immunoassay Analyzer for details.

“Quick test” mode: Insert the test cassette into the Analyzer at 15 minutes after sample

application and click "New test ", the Analyzer will automatically give the test result after a

few seconds.

“Standard test” mode: Insert the test cassette into the Analyzer immediately after sample

application, click "New test" at the same time, the Analyzer will automatically count down 15

minutes. After the countdown, the Analyzer will give the result at once.

【INTERPRETATION OF RESULTS】

Results read by Fluorescence Immunoassay Analyzer.

The result of tests for cTnT is calculated by Fluorescence Immunoassay Analyzer

and display the result on the screen. For additional information, please refer to the user manual

of Fluorescence Immunoassay Analyzer.

Linearity range of cTnT Test is 0.2-40 ng/mL.

Tags:

rapid diagnostic test kits

One Step Rapid Test

home testing kits

High Specificity Fluorescence Immunoassay Test Kits THC Test Cassette

Get Best Price

Fluorescence Immunoassay OPI Test Cassette For Urine Detection

Get Best Price

High Sensitivity MTD Test Cassette Fluorescence Immunoassay Test

Get Best Price

High Specificity Fluorescence Immunoassay Test Kits For Drug Abuse

Get Best Price

High Sensitivity Fluorescence Immunoassay Kit For Sensitive Drug Detection

Get Best Price

HIgh Sensitivity Fluorescence Immunoassay Test Kits Oxazepam Urine Type Test Kit

Get Best Price

High Accuracy Fluorescence Immunoassay Test Kit For BAR Detention Drug Of Abuse

Get Best Price

AMP Test Cassette Fluorescence Immunoassay Test Kit

Get Best Price

Custom Monoclonal Antibody

Drug Abuse Test Kit

Rapid Test Kit

Infectious Disease Testing

Accuate Cardiac Marker Rapid Test kits

Convenient Oncology Rapid Diagnostic Test kits

Rapid Diagnostic Test Kits

Fluorescence Immunoassay Analyzer

Glucose Monitoring Device

Veterinary Rapid Test Kits

Flourescence Immunoassay Test Kits

Urine Chemistry Analyzer

Nucleic Acid PCR

Rapid Test Reader

Chemiluminescence Immunoassay Test

Blood Biochemistry Analyzer

Custom Monoclonal Antibody

Drug Abuse Test Kit

Rapid Test Kit

Infectious Disease Testing

Accuate Cardiac Marker Rapid Test kits

Convenient Oncology Rapid Diagnostic Test kits

Rapid Diagnostic Test Kits

Fluorescence Immunoassay Analyzer

Glucose Monitoring Device

Veterinary Rapid Test Kits

Flourescence Immunoassay Test Kits

Urine Chemistry Analyzer

Nucleic Acid PCR

Rapid Test Reader

Chemiluminescence Immunoassay Test

Blood Biochemistry Analyzer

products details

Flourescence Immunoassay Test cassette for Cardiac Troponin T Detection in Whole Blood/Serum/Plasma

Flourescence Immunoassay Test cassette for Cardiac Troponin T Detection in Whole Blood/Serum/Plasma

mouse monoclonal antibodies

hybridoma Monoclonal antibodies