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Quantitative Cortisol Test Cassette Fluorescence Immunoassay for Human Serum/Plasma

Name: Quantitative Cortisol Test Cassette Fluorescence Immunoassay for Human Serum/Plasma
Brand: Hangzhou AllTest Biotech CO.,LTD
SKU: FI-COR-302
Price: 0.25 USD
Availability: InStock

Brand Name:	ALLTEST Novatrend Plus
Model Number:	FI-COR-302
MOQ:	N/A
Price:	negotiation
Payment Terms:	Ex works

Detail Information

Product Description

Detail Information

Accuracy:

High

Cat No:

FI-COR-302

Sample:

Serum/plasma

Time To Results:

15 Minutes

Storage Temperature:

4-30℃

Specificity:

High

Usage:

In Vitro Diagnostic Use

Intended Use:

For In Vitro Diagnostic Use Only

Highlight:

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Product Description

The Cortisol Test Cassette (Serum/Plasma) is based on fluorescence immunoassay for

the quantitative determination of cortisol in human serum, plasma with the use of

fluorescence immunoassay analyzer. It is intended to be used as an aid in the

diagnosis of related diseases with abnormal cortisol levels.

【SUMMARY】

Cortisol is a hormone, one of the glucocorticoids, made in the cortex of the

adrenal glands and then released into the blood, which transports it all round the body.

Almost every cell contains receptors for cortisol and so cortisol can have lots of different

actions depending on which sort of cells it is acting upon. These effects include

controlling the body’s blood sugar levels and thus regulating metabolism, acting as an

anti-inflammatory, influencing memory formation, controlling salt and water balance,

influencing blood pressure and helping development of the foetus. In many species

cortisol is also responsible for triggering the processes involved in giving birth.

A similar version of this hormone, known as corticosterone, is produced by rodents,

birds and reptiles.

【PRINCIPLE】

The Cortisol Test Cassette (Serum/Plasma) detects cortisol based on Fluorescence

Immunoassay. The specimen moves through the strip from sample pad to absorbent

pad. Cortisol in the specimen will compete with the cortisol antigen coated on the

membrane. The less cortisol in the specimen, the more chance that fluorescent

microspheres-conjugated anti-cortisol antibodies can be captured by the cortisol

antigen coated on the membrane (Test line). The concentration of cortisol in the

specimen is inversely related to the intensity of the fluorescent signal captured on the T

line. According to the fluorescence intensity of the test and the standard curve, the

concentration of cortisol in the specimen can be calculated by analyzer to show cortisol

concentration in specimen.

【REAGENTS】

The test includes anti-cortisol antibody coated fluorophore and Cortisol-BSA coated on

the membrane.

【PRECAUTIONS】

1. For professional in vitro diagnostic use only.

2. Do not use after the expiration date indicated on the package. Do not use the test if

the foil pouch is damaged. Do not reuse.

3. Avoid cross-contamination of specimens by using a new specimen collection

container for each specimen obtained.

4. Do not eat, drink or smoke in the area where the specimens and tests are handled.

Handle all specimens as if they contain infectious agents. Observe established

precautions against microbiological hazards throughout the procedure and follow

standard procedures for proper disposal of specimens. Wear protective clothing

such as laboratory coats, disposable gloves and eye protection when specimens

are assayed.

5. Do not interchange or mix reagents from different lots.

6. Humidity and temperature can adversely affect results.

7. Used testing materials should be discarded in accordance with local regulations.

8. Read the entire procedure carefully prior to any testing.

9. The Cortisol Test Cassette should only be used with the analyzer by medical

professionals.

【STORAGE AND STABILITY】

1. The test should be stored at 4-30 °C until the expiration date printed on the sealed

pouch.

2. The test must remain in the sealed pouch until use.

3. Do not freeze.

4. Care should be taken to protect the components of the test from contamination.

5. Do not use if there is evidence of microbial contamination or precipitation. Biological

contamination of dispensing equipment, containers or reagents can lead to false

results.

【SPECIMEN COLLECTION AND PREPARATION】

1. Collect the specimens according to standard procedures.

2. Do not leave specimens at room temperature for prolonged periods. Serum and

plasma specimens may be stored at 2-8 °C for up to 3 days, for long term storage,

specimens should be kept below -20 °C.

3. Bring specimens to room temperature prior to testing. Frozen specimens must be

completely thawed and mixed well prior to testing. Avoid repeated freezing and

thawing of specimens. Only clear, non-hemolyzed specimens can be used.

4. EDTA K2 can be used as the anticoagulant tube for collecting the blood specimen.

【MATERIALS】

Materials Provided

• Test Cassettes

• Specimen Collection Tubes with Buffer

• ID Card

• Package Insert

Materials Required But Not Provided

• Timer

• Centrifuge

• Fluorescence Immunoassay Analyzer

• Pipette

• Specimen Collection Containers

【DIRECTIONS FOR USE】

Refer to Fluorescence Immunoassay Analyzer User Manual for the complete

instructions on use of the analyzer. The test should be conducted at room temperature.

Allow the test, specimen and buffer to reach room temperature (15-30 °C) prior to

testing.

1. Turn on the Analyzer power.

2. Take out the ID card and insert it into the ID Card Slot. Choose test mode and/or

sample type according to needs.

3. Remove the test cassette from the sealed foil pouch and use it within 1 hour. Best

results will be obtained if the assay is performed immediately after opening the foil

pouch.

4. Place the test on a flat and clean surface.

Pipette 75 μL of serum or plasma into the specimen collection tube with buffer;

mix the specimen and the buffer well.

Note: The diluted specimen should be used immediately or stored for up to 4

hours at 2-8 °C.

5. Pipette 75 μL of diluted specimen into the sample well of the cassette. Start the

timer at the same time.

6. Test results should be interpreted at 15 minutes with the use of Fluorescence

Immunoassay Analyzer.

Caution: There are different test modes of the Fluorescence Immunoassay Analyzer.

The difference between them is incubation of the test cassette is outside or inside the

analyzer. Choose test mode accordingly and confirm sample type. Consult the user

manual of the analyzer for detailed operation information.

Operator must consult the Fluorescence Immunoassay Analyzer User Manual prior to

use and become familiar with the processes and quality control procedures.

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products details

Quantitative Cortisol Test Cassette Fluorescence Immunoassay for Human Serum/Plasma

Quantitative Cortisol Test Cassette Fluorescence Immunoassay for Human Serum/Plasma

mouse mab

mono clonal antibody