CDV +CPV Ab Combined Rapid Test Convenient Use Rapid Assay Test
The CDV+CPV Antibody Combo Rapid Test Cassette (Serum/Plasma) is a combined cassette for semi-quantitative detection of Canine Distemper Virus Antibody and Canine Parvovirus antibody in canine’s serum or plasma.
The CDV+CPV Antibody Combo Rapid Test Cassette (Serum/Plasma) is based on sandwich lateral flow immunochromatographic assay. The test cassette has twotesting windows. Each testing window has an invisible T (test) zone and a C (control) zone. When the sample was applied into the sample well(S) on the cassette, the liquid will laterally flow through the surface of the test strip .If there are enough CDV antibody or CPV antibody in the specimen, a visible T line will appears. The C line should always appear after a sample is applied, which indicates a valid result.
How to use?
Allow the test cassette, specimen, buffer, and/or controls to equilibrate to room temperature (15-30 °C) prior to testing.
1. Remove the test cassette from the foil pouch and use it as soon as possible. Best results will be obtained if the assay is performed within one hour.
2. Collect canine’s fresh whole blood, and separate serum or plasma from blood as soon as possible to avoid hemolysis. Only use clear, non-hemolyzed specimens. Do not leave the specimens at room temperature for prolonged periods. Serum and plasma specimens may be stored at 2-8 °C for up to 3 days. For long term storage, specimens should be kept below -20 °C.
3. Place the test cassette on a clean and level surface. Hold the dropper vertically and transfer 1 drop of serum or plasma (approximately 40 μL) and immediately add 2 drops of buffer (approximately 80μL) into each specimen well(S) separately, then start the timer. See illustration below.
4. Read the results in 10 minutes by comparing the T line intensity with the color card provided. Do not interpret results after 15 minutes.
The presence of “C” line indicates a valid result. Read the result by comparing the color intensity of the “T” line with the standard color card provided.
For CDV antibody:
The V.N. titers of canine distemper antibodies are corresponding with the color intensity grades.
G1 < 1:8 G2 = 1:16 G3 = 1:32
G4 = 1:64 G5 = 1:128 G6 > 1:256
Positive: The presence of both “C” line and “T” line. T line at G3 is considered as standard positive. T line < G3, is weak positive and indicates low CDV antibody level. T line around G4- G5, is strong positive. T line > G6, is very strong positive, indicates a contact with virus recently or in infection.
Negative: Clear C line appears. No T line, or T line is weaker than G1. It indicates very low CDV titer. Failed
immunization may happen.
Invalid: No line appears in control line zone (C).
For CPV antibody:
The H.I. titers of canine parvo virus antibodies are corresponding with the color intensity grades.
G1 < 1:40 G2 = 1:40 G3 = 1:80
G4 = 1:160 G5 = 1:320 G6 > 1:640
Positive: The presence of both “C” line and “T” line. T line intensity higher than G3 is considered as positive. T line < G3, is weak positive and indicates low CPV antibody level. T line around G4-G5 is strong positive. T line > G6 is very strong positive, indicates a contact with virus recently or in infection.
Negative: Clear C line appears. No T line, or T line is weaker than G1. It indicates very low CPV titer. Failed immunization may happen.
Invalid: No colored line appears in C zone.