The CEA Test Cassette (Serum/Plasma) is based on Fluorescence immunoassay for the quantitative detection of CEA in serum or plasma to aid in monitoring of cancer patients.
Carcinoembryonic Antigen (CEA) is a tumor-associated antigen characterized as an oncofetal glycoprotein. CEA is expressed in a variety of malignancies, particularly pulmonary or gastrointestinal tumors (e.g. colon cancer, liver cancer and lung cancer). CEA normally occurs in fetal gut tissue with detectable serum levels essentially disappearing after birth. Therefore, elevated levels of CEA can be of significant value in the diagnosis of primary carcinomas. In addition to quantitative assessment, CEA testing plays an important role in the monitoring of cancer patients. Clinical evidence indicates that CEA levels can serve as predictive markers in both pre- and post-treatment of cancer. Progressive elevation of CEA may signal tumor recurrence 3-36 months before clinical evidence of metastasis. Persistent elevation of circulating CEA following treatment is strongly indicative of occult metastatic and residual diseases and deficient therapeutic response.
Refer to Fiatest TM Go Fluorescence Immunoassay Analyzer Operation Manual for the complete instructions on use of the Test. The test should be in room temperature.
Allow the test, specimen, buffer and/or controls to reach room temperature (15-30°C) prior to testing.
1. Turn on the Analyzer power. Then according to the need, select “standard test” or “Quick test” mode.
2. Remove the ID card and insert it into the Analyzer ID Card Slot.
3. Pipette 50 μLof serum or plasma into the buffer tube, mix the specimen and the buffer well.
4. Pipette 75 μL diluted sample into the sample well of the cassette. Start the timer at the same time.
5. There are two test modes for Fiatest TM Go Fluorescence Immunoassay Analyzer, Standard Test mode and Quick Test mode. Please refer to the user manual of Fiatest TM Go Fluorescence Immunoassay Analyzer for details.
“Quick test” mode: After 15 minutes of adding sample,Insert the test cassette into the Analyzer click “QUICK TEST”, fill the test information and click "NEW TEST" immediately. The Analyzer will automatically give the test result after a few seconds.
“Standard test” mode: Insert the test cassette into the Analyzer immediately after
adding specimen, click “STANDARD TEST”, fill the test information and click "NEW TEST" at the same time. The Analyzer will automatically countdown 15 minutes. After the countdown, the Analyzer will give the result at once.
INTERPRETATION OF RESULTS
Results read by Fiatest TM Go Fluorescence Immunoassay Analyzer.
The result of tests for CEA is calculated by FluroLit TM Fluorescence Immunoassay Analyzer and display the result on the screen. For additional information, please refer to the user manual of Fiatest TM Go.
Fluorescence Immunoassay Analyzer.
Linearity range of FluroLit TM CEA is 1-500 ng/mL.
||CEA Test Cassette