AllTest Calprotectin Rapid Test Cassette In Human Feces Specimen , 10T / Kit

Basic Information
Place of Origin: CHINA
Brand Name: AllTest
Certification: CE
Model Number: cassette
Minimum Order Quantity: 500
Packaging Details: 10T/Kit
Supply Ability: 100 Million a year
Format: Cassette Specimen: Feces
Kit Size: 10T/Kit Cut-Off: See Insert
Storage: 2-30℃ Shelf Time: 24 Months
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AllTest Calprotectin Rapid Test Cassette In Human Feces Specimen , 10T / Kit

 

 

Applications:

 

The Calprotectin Rapid Test Cassette (Feces) is a rapid chromatographic immunoassay for the qualitative detection of Calprotectin in human feces specimen which might be useful for the diagnosis of inflammatory gastrointestinal disorders.

 

 

Description:

 

Calprotectin is a 24 kDa dimer of calcium binding proteins S100A8 and S100A9.The complex accounts for up to 60% of the soluble protein content of the neutrophil cytosol.Calprotectin becomes available in the intestinal lumen via leukocyte shedding,active secretion,cell disturbance, and cell death.This results in elevated faecal calprotectin levels, which can be detected in the stool.Elevated faecal calprotectin levels therefore indicate migration of neutrophils into the intestinal mucosa, which occurs during intestinal inflammation.Faecal calprotectin has been used to detect intestinal inflammation, and can serve as a marker for inflammatory bowel diseases.Calprotectin is useful as a marker, as it is resistant to enzymatic degradation, and can be easily measured in faeces.

 

 

How to use?

 

Allow the test, specimen, buffer and/or controls to reach room temperature (15-30°C) prior to testing.

Collect sufficient quantity of feces (1-2 mL or 1-2 g) in a clean, dry specimen collection container to obtain maximum antigens (if present). Best results will be obtained if the assay is performed within 6 hours after collection. Specimen collected may be stored for 3 days at 2-8℃ if not tested within 6 hours. For long term storage, specimens should be kept below -20℃.

• For Solid Specimens:

Unscrew the cap of the specimen collection tube,then randomly stab the specimen collection applicator into the fecal specimen in at least 3 different sites to collect approximately 50 mg of feces (equivalent to 1/4 of a pea). Do not scoop the fecal specimen.

• For Liquid Specimens:

Hold the dropper vertically, aspirate fecal specimens, and then transfer 2 drops (approximately 80 μL) into the specimen collection tube containing the extraction buffer.

Note: If the specimen does not migrate (presence of particles), centrifuge the extracted specimens contained in the extraction buffer vial. Collect 80 μL of supernatant, dispense into the specimen well (S) of a new test cassette and start afresh following the instructions mentioned above.AllTest Calprotectin Rapid Test Cassette In Human Feces Specimen , 10T / Kit 0

 

 

INTERPRETATION OF RESULTS

(Please refer to the illustration above)

POSITIVE:* Two lines appear. One colored line should be in the control line region (C) and another apparent colored line should be in the test line region (T).

*NOTE: The intensity of the color in the test line region (T) will vary depending on the concentration of Calprotectin present in the specimen. Therefore, any shade of color in the test line region (T) should be considered positive.

 

NEGATIVE: One colored line appears in the control line region (C). No line appears in the test line region (T).

 

INVALID: Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test with a new test. If the problem persists, discontinue using the test kit immediately and contact your local distributor.

 

 

QUALITY CONTROL

 

Internal procedural controls are included in the test. A colored line appearing in the control region (C) is an internal valid procedural control. It confirms sufficient specimen volume and correct procedural technique.

Control standards are not supplied with this kit; however, it is recommended that positive and negative controls be tested as a good laboratory practice to confirm the test procedure and to verify proper test performance.

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