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|Format:||Cassette||Specimen:||WB /S/ P|
|Storage:||2-30℃||Shelf Time:||24 Months|
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Fast reading SAA Rapid Test Cassette Rapid Test CE Approve
The SAA Rapid Test Cassette (Whole Blood/Serum/Plasma) is a rapid chromatographic immunoassay for the qualitative detection of human SAA in whole blood, serum or plasma as an aid in the diagnosis of inflammatory conditions. The cut-off of the test is 10μg/ml.
Serum amyloid A (SAA) proteins are a family of apolipoproteins associated with high-density lipoprotein (HDL) in plasma. Different isoforms of SAA are expressed constitutively (constitutive SAAs) at different levels or in response to inflammatory stimuli (acute phase SAAs). These proteins are produced predominantly by the liver. The conservation of these proteins throughout invertebrates and vertebrates suggests that SAAs play a highly essential role in all animals. Acute-phase serum amyloid A proteins (A-SAAs) are secreted during the acute phase of inflammation. These proteins have several roles, including the transport of cholesterol to the liver for secretion into the bile, the recruitment of immune cells to inflammatory sites, and the induction of enzymes that degrade extracellular matrix. Serum amyloid A (SAA) is also an acute phase marker that responds rapidly. Similar to CRP, levels of acute-phase SAA increase within hours after inflammatory stimulus, and the magnitude of increase may be greater than that of CRP. Relatively trivial inflammatory stimuli can lead to SAA responses. It has been suggested that SAA levels correlate better with disease activity in early inflammatory joint disease than do ESR and CRP. Although largely produced by hepatocytes, more recent studies show that SAA is produced by adipocytes as well, and its serum concentration is associated with body mass index.
How to use?
Bring tests, specimens, buffer, and/or controls to room temperature (15-30°C) before use.
1. Remove the Test Cassette from its sealed pouch, and place it on a clean, level surface. For best results, the assay should be performed within one hour.
2. Open the tube of the diluted sample .Transfer 3 drops of diluted sample(approximately 120 μL)to sample well. Start the timer.
3. Wait for the colored lines to appear. The result should be read at 5 minutes. Do not interpret the results after 10 minutes.
【INTERPRETATION OF RESULTS 】
POSITIVE:* Two lines appear. One colored line should be in the control line region (C) and another apparent colored line should be in the test line region (T).
*NOTE: The intensity of the color in the test line region (T) will vary depending on the concentration of SAA antigen present in the specimen. Therefore, any shade of color in the test line region (T) should be considered positive.
NEGATIVE: One colored line appears in the control line region (C). No line appears in the test
line region (T).
INVALID: Control line fails to appear. Insufficient specimen volume or incorrect procedural
techniques are the most likely reasons for control line failure. Review the procedure and repeat the
test with a new test. If the problem persists, discontinue using the test kit immediately and contact
your local distributor.
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