Allow the test cassette, reagents, throat swab specimen, and/or controls to reach room temperature (15-30℃) prior to testing.
1.Exact QR Code from the QR code card for specific information of the test. Use only QR code card provided inside the test kits.
2.Remove the test cassette from the sealed foil pouch and use it within one hour. Best results will be obtained if the test is performed immediately after opening the foil pouch.
3.Hold the Extraction Reagent 1 bottle vertically and add 4 full drops (approximately 240μL) of Extraction Reagent 1 to an extraction tube. Extraction Reagent 1 is red in color. Hold the Extraction Reagent 2 bottle vertically and add 4 full drops (approximately 160μL) to the tube. Extraction Reagent 2 is colorless. Mix the solution by gently swirling the extraction tube. The addition of Extraction Reagent 2 to Extraction Reagent 1 changes the color of the solution from red to yellow. See illustration 1.
4.Immediately add the swab into the extraction tube, agitate the swab vigorously 15 times, leave the swab in the extraction test tube for 1 minute. See illustration 2
5.Press the swab against the side of the tube and squeeze the bottom of the tube while removing the swab so that most of the liquid stays in the tube. Discard the swab. See illustration 3
6.Fit the dropper tip onto top of the extraction tube. Place the test cassette on a clean and level surface. Add 3 drops of the solution (approx.100μl) to the sample well and then start the timer. See illustration 4 and illustration 5.
7.Wait for the colored line(s) to appear. The result should be read with AllTest™ LF Reader at 5 minutes.
8.Do not interpret test results visually.
9.For Alltest™ LF Reader installation, start up and complete instructions refer to the Alltest™ LF Reader User Manual. Operator must consult the Alltest™ LF Reader User Manual prior to use and become familiar with the processes and quality control procedures.
Note: For the Alltest™ LF Reader installation, start up and complete instructions refer to the Alltest™ LF Reader User Manual. Operator must consult the Alltest™ LF Reader User Manual prior to use and become familiar with the processes and quality control procedures.
Note: It is suggested not to use the buffer beyond 30 days after opening.
INTERPRETATION OF RESULTS
Results read by Alltest LF reader
The result of positive or negative for Group A Streptococcal infection is determined by the Alltest LF reader.
Result could be exported to excel.