Chikungunya IgG/IgM Rapid Test Cassette (Serum/Plasma)

A rapid test for the qualitative detection of IgG and IgM antibodies to Chikungunya in human serum or plasma For professional in vitro diagnostic use only

Applications:

The Chikungunya IgG/IgM Rapid Test Cassette (Serum/Plasma) is a rapid chromatographic immunoassay for the qualitative detection of IgG and IgM antibodies to Chikungunya in human’s serum or plasma. It is intended to be used as a screening test and as an aid in the diagnosis of infection with CHIK. Any reactive specimen with the Chikungunya IgG/IgM Rapid Test must be confirmed with alternative testing method(s) and clinical findings.

Description:

Chikungunya is a rare viral infection transmitted by the bite of an infected Aedes aegypti mosquito. It is characterized by a rash, fever, and severe joint pain (arthralgias) that usually lasts for three to seven days. The name is derived from the Makonde word meaning ‘that which bends up’ in reference to the stooped posture developed as a result of the arthritic symptoms of the disease. It occurs during the rainy season in tropical areas of the world,primarily in Africa, South-East Asia, southern India and Pakistan. The symptoms are most often clinically indistinguishable form those observed in dengue fever. Indeed, dual infection of dengue and chikungunya has been reported in India. Unlike dengue, hemorrhagic manifestations are relatively rare and most often the disease is a self limiting febrile illness. Therefore it is very important to clinically distinguish dengue from CHIK infection. CHIK is diagnosed based on serological analysis and viral isolation in mice or tissue culture. An IgM immunoassay is the most practical lab test method.

How to use?

a. Bring the pouch to room temperature before opening it. Remove the test cassette from the sealed pouch and use it as soon as possible. Best results will be obtained if the assay is performed within one hour.
b. Place the test cassette on a clean and level surface. Hold the dropper vertically and transfer 1 drop of serum or plasma (40μl) to the specimen well of the test cassette, then add 2 drops of buffer (80μl) * and start the timer. Avoid trapping air bubbles in the specimen well. See the illustration below.
c. Wait for the colored line(s) to appear. Read the result at 15 minutes, do not interpret the result after 20 minutes.
* NOTE: It should be add one more drop of (approx. 40ul) buffer to the sample well of the test cassette if the specimen runs too slowly.