A rapid test for the qualitative detection of 3, 4-methylenedioxypyrovalerone in human urine that can be performed with the use of AllTestTM LF Reader. For medical and other professional in vitro diagnostic use only.
The MDPV Rapid Test Cassette (Urine) is a rapid chromatographic immunoassay for the detection of 3, 4-methylenedioxypyrovalerone in human urine that can be performed with the use of LF Reader at a cut-off concentration of 1000ng/mL.
This assay provides only a qualitative, preliminary analytical test result. A more specific alternate chemical method must be used in order to obtain a confirmed analytical result. Gas chromatography/mass spectrometry (GC/MS) or Liquid Chromatography/mass spectrometry (LC/MS) are the preferred confirmatory methods. Clinical consideration and professional judgment should be applied to any drug of abuse test result, particularly when preliminary positive results are used.
3, 4-methylenedioxypyrovalerone (MDPV) is a psychoactive recreational drug with stimulant properties which acts as a norepinephrine-dopamine reuptake inhibitor (NDRI). It was first developed in the 1960s by a team at BoehringerIngelheim. MDPV remained an obscure stimulant until around 2004 when it was reportedly sold as a designer drug. Products labeled as bath salts containing MDPV were previously sold as recreational drugs in gas stations and convenience stores in the United States, similar to the marketing for Spice and K2 as incense.
MDPV is the 3,4-methylenedioxy ring-substituted analog of the compound pyrovalerone, developed in the 1960s, which has been used for the treatment of chronic fatigue and as an anorectic, but caused problems of abuse and dependence. However, despite its structural similarity, the effects of MDPV bear little resemblance to other methylenedioxyphenylalkylamine derivatives such as 3,4-methylenedioxy-N-methylamphetamine (MDMA), instead producing primarily stimulant effects with only mild entactogenic qualities.
MDPV undergoes CYP450 2D6, 2C19, 1A2, and COMT phase 1 metabolism (liver) into methylcatechol and pyrrolidine, which in turn are glucuronated (uridine 5'-diphospho-glucuronosyl-transferase) allowing it to be excreted by the kidneys, with only a small fraction of the metabolites being excreted into the stools. No free pyrrolidine will be detected in the urine.
The MDPV Rapid Test Cassette (Urine) is a rapid urine screening test that can be performed with the use of LF Reader. The test utilizes a monoclonal antibody to selectively detect elevated levels of 3,4-methylenedioxypyrovalerone in urine. The MDPV Rapid Test Cassette (Urine) yields a positive result when 3,4-methylenedioxypyrovalerone in urine exceeds 1000ng/mL.
Allow the test, urine specimen, and/or controls to reach room temperature (15-30C) prior to testing.
1.Exact QR Code from the QR code card for specific information of the test. Use only QR code card provided inside the test kits.
2.Bring the pouch to room temperature before opening it. Remove the test cassette from the sealed pouch and use it within one hour.
3.Place the test cassette on a clean and level surface. Hold the dropper vertically and transfer 3 full drops of urine (approx. 120μL) to the specimen well (S) of the test cassette, and then start the timer. Avoid trapping air bubbles in the specimen well (S). See the illustration below.
4.Wait for the colored line(s) to appear. The Results should be Read with AllTestTM LF Reader at 5 minutes.
5.Do not interpret test results visually.
INTERPRETATION OF RESULTS
Results read by AllTestTM LF Reader
The result of positive or negative for each analyte is determined by AllTestTM LF Reader.
Result may be exported to excel or PDF.
At this point the Test Cassette may be removed and appropriately discarded.
||Methylenedioxypyrovalerone (MDPV) Rapid Test Cassette