products details

Home Products

Serum Amyloid A Protein SAA Test Kit CLIA Non Specific Inflammatory Indicator

Name: Serum Amyloid A Protein SAA Test Kit CLIA Non Specific Inflammatory Indicator
Brand: Hangzhou AllTest Biotech CO.,LTD
SKU: CI-SAA
Price: Negotiable USD
Availability: InStock

Brand Name:	Unveil
Model Number:	CI-SAA
MOQ:	N/A
Price:	negotiation
Supply Ability:	10 M tests/month

Detail Information

Product Description

Detail Information

Place of Origin:

China

Certification:

Product Name:

Serum Amyloid A Protein (SAA) Test Kit (CLIA)，non-specific Inflammatory Indicator， Chemiluminescence Immunoassay

Cat No.:

CI-SAA

Principle:

Double Antibody Sandwich Method

Specimen:

WB/S/P

Pack:

40T

Shelf Life:

2 Years

Format:

Strip

Certificate:

Storage Temperature:

2-8℃

Cut-Off:

1-500 μg/mL

Packaging Details:

40T

Supply Ability:

10 M tests/month

Highlight:

Serum Amyloid A Protein Test Kit

SAA Chemiluminescence Immunoassay Test

Product Description

Serum Amyloid A Protein (SAA) Test Kit (CLIA),non-specific inflammatory indicator, Chemiluminescence Immunoassay

Product Name:	Serum Amyloid A Protein (SAA) Test Kit (CLIA),non-specific Inflammatory Indicator, Chemiluminescence Immunoassay
Principle:	Double Antibody Sandwich Method
Pack:	40T
Format:	Strip
Storage Temperature:	2-8℃
Cat No.:	CI-SAA
Specimen:	WB/S/P
Shelf Life:	2 Years
Certificate:	CE
Cut-Off:	1-500 μg/mL

A CLIA test kit for the quantitative determination of Serum Amyloid A Protein (SAA) in human whole blood, serum or plasma with the use of Automatic Chemiluminescence Immunoassay Analyzer.

【INTENDED USE】

The Serum Amyloid A Protein (SAA) Test Kit (CLIA) is intended for quantitative determination of Serum Amyloid A Protein (SAA) in human whole blood, serum and plasma and mainly used as a non-specific inflammatory indicator.

For professional in vitro diagnostic use only.

【SUMMARY】

Serum amyloid A is an acute-phase protein, which is used in inflammation, infection and non-infectious during infectious diseases, its concentration in the blood can rise sharply within a few hours. It can be increased to 1000 times the initial concentration.

Serum amyloid A is related to high-density lipoprotein (HDL), which regulates the metabolism of high-density lipoprotein during inflammation. The SAA family includes different expression forms of apolipoprotein, acute SAA (A-SAAs) and structural SAA (C-SAAs).

A particularly important feature of serum amyloid A is that its degradation products can be converted into starch like protein A (AA) fibrils deposited in different organs, in chronic inflammation. This is a serious complication in severe disease.Similar to C-reactive protein (CRP), the detection of serum amyloid A can help diagnose inflammation, assess and monitor the activity and treat it.

However, the serum amyloid A test is more effective than the C-reactive protein test in the diagnosis of viral infections, renal transplant rejection patients (especially those undergoing immunosuppressive therapy) and cystic fibrosis patients treated with adrenocortical hormones.

【PRINCIPLE】

This product uses the double antibody sandwich method. In the first step, the sample, the SAA antibody labeled with alkaline phosphatase and the magnetic particles coated with the SAA antibody are mixed. After incubation, the SAA in the sample forms an immune complex with the corresponding antibody. In the second step, magnetic separation and cleaning are performed to remove free enzyme-labeled antibodies. The third step is to add the chemiluminescent substrate solution to the immune complex. Luminescence signal generated by the enzyme reaction is detected by Automatic Chemiluminescence Immunoassay Analyzer and the detected luminescence intensity is related to the concentration of SAA in the sample. Automatic Chemiluminescence Immunoassay Analyzer can calculate the concentration of SAA in the sample.

【REAGENTS】

The reagent strip includes SAA antibody coated with magnetic particles, alkaline phosphatase labeled SAA antibody, wash buffer and substrate solution.

【SPECIMEN COLLECTION AND PREPARATION】

Collect and handle the specimen strictly as below:

1. Collect the specimen according to standard procedures.

2. Human serum collected using standard sampling tubes or tubes containing separating gel.

3. Human whole blood and plasma are collected using EDTA or Heparin sodium.

4. Separate human serum and plasma from blood as soon as possible to avoid hemolysis. Grossly hemolytic, lipidic or turbid samples should not be used. Specimen with extensive particulate should be clarified by centrifugation prior to use. Do not use specimens with fibrin particles or contaminated with microbial growth.

5. Do not leave specimens at room temperature for prolonged periods.

Serum and Plasma specimens may be stored at 2-8 °C for up to 3 days, below -20 °C stable for 3 months. Whole blood collected by venipuncture should be stored at 2 -8 °C if the test is to be used within 1 day of collection. Do not freeze whole blood specimens.

6. Bring specimens to room temperature prior to testing. Frozen specimens must be completely thawed and mixed well prior to testing. Specimens should not be frozen and thawed repeatedly.

7. If the patients had been treated by high dose of biotin (5 mg/day), blood collection must be done after at least 8 hours.

8. If specimens are to be shipped, pack them in compliance with local regulations covering the transportation of etiologic agents.

【DIRECTIONS FOR USE】

1. Preparation

1) Take the test kit from the refrigerated environment and use it after

equilibrating to room temperature.

2) Dissolution of the Calibrator and Control:

2. Calibration

1) When using a new batch of reagents, calibration must be priority performed after the reagent information is entered.

2) The system requires double parallel tests for calibrator 1 and calibrator 2 and will assign 2 test slots to calibrator 1 and calibrator 2 respectively by default and the four tests should be done in one time.

Follow calibration procedures according to the user manual of the analyzer.

Load the reagent strips, then add 100-150 μL of corresponding dissolved calibrator 1 and 2 to the corresponding sample well of the reagent strips, finally tap the start button to perform the calibration tests.

3) Calibration frequency

3. Quality Control

1) Input Quality control information

Input the QC information into the system by scanning the QR code of the quality control in the information card provided with the kit.

2) Follow control procedures according to the user manual of the analyzer.

3) Load the reagent strips and add 100-150 μL of corresponding dissolved control materials, then tap the start button to perform the control tests. The recommended control requirement for the SAA test is a single test of all control levels tested once every 24 hours in daily use. If the quality control procedures in your laboratory require more frequent use of controls to verify test results, follow your laboratory-specific procedures. Make sure the control values are within the defined ranges.

Each laboratory should establish corrective measures to be taken if control values fall outside the defined limits.

4. Sample test

Sample test can be performed on condition that the test results of calibration and quality control meet the requirements. (Calibration results indicate successful and Control results fall within the specified ranges in the information card.)

Dilution of the sample: pipette 2 μL of sample to the diluent provided with the kit, shake to mix thoroughly.

Tap [Analy] and select [SAA] item.

Load the reagent strips and add 100-150 μL of diluted sample to the sample well, and tap Start button. After the detection is finished, the concentration will be calculated by the software and displayed on the screen.

Note: the diluted sample should be used within 24 hours.

5. Dilution

Specimens with a SAA value exceeding 500 μg/mL are flagged with the code “>500.00” and may be diluted again and retest.

50mM Tris buffer is recommended as sample diluents. Note: After manual dilution, the dilution factor should be considered when calculate the final results. For detailed information, please refer to the User Manual of Automatic Chemiluminescence Immunoassay Analyzer

Tags:

Custom Monoclonal Antibody

Drug Abuse Test Kit

Rapid Test Kit

Infectious Disease Testing

Accuate Cardiac Marker Rapid Test kits

Convenient Oncology Rapid Diagnostic Test kits

Rapid Diagnostic Test Kits

Fluorescence Immunoassay Analyzer

Glucose Monitoring Device

Veterinary Rapid Test Kits

Flourescence Immunoassay Test Kits

Urine Chemistry Analyzer

Nucleic Acid PCR

Rapid Test Reader

Chemiluminescence Immunoassay Test

Custom Monoclonal Antibody

Drug Abuse Test Kit

Rapid Test Kit

Infectious Disease Testing

Accuate Cardiac Marker Rapid Test kits

Convenient Oncology Rapid Diagnostic Test kits

Rapid Diagnostic Test Kits

Fluorescence Immunoassay Analyzer

Glucose Monitoring Device

Veterinary Rapid Test Kits

Flourescence Immunoassay Test Kits

Urine Chemistry Analyzer

Nucleic Acid PCR

Rapid Test Reader

Chemiluminescence Immunoassay Test

products details

Serum Amyloid A Protein SAA Test Kit CLIA Non Specific Inflammatory Indicator

Serum Amyloid A Protein SAA Test Kit CLIA Non Specific Inflammatory Indicator

Serum Amyloid A Protein Test Kit

SAA Chemiluminescence Immunoassay Test