Infectious Mononucleosis Test / Heterophile Antibody Test cassette

Applications: 

The MONO Rapid Test Cassette (Whole Blood/Serum/Plasma) is a rapid chromatographic immunoassay for the qualitative detection of Infectious Mononucleosis heterophile antibodies in whole blood, serum or plasma as an aid in the diagnosis of Infectious Mononucleosis.

Description: 

Infectious Mononucleosis (IM) is caused by the Epstein-Barr virus, which is a member of the herpesvirus family. Symptoms of IM are fever, sore throat and swollen lymph glands. In very rare cases, heart or central nervous system problems may occur. Diagnosis of IM is made based on the presence of heterophile antibodies. Infectious Mononucleosis heterophile antibodies belong to the IgM class. They are present in 80-90% of acute IM cases and can be detected in 60-70% of patients during the first week of clinical illness.^1,2,3,4

The MONO Rapid Test Cassette (Whole Blood/Serum/Plasma) is a simple test that utilizes an extract of bovine erythrocytes to qualitatively and selectively detect Infectious Mononucleosis heterophile antibodies in whole blood, serum or plasma in minutes.

How to use? 

Allow the test, specimen, buffer, and/or controls to equilibrate to room temperature (15‑30°C) prior to testing.

1. Remove the Test Cassette from the foil pouch and use it as soon as possible. Best results will be obtained if the assay is performed within one hour.
2. Place the Test Cassette on a clean and level surface.

For Serum or Plasma specimens:

Hold the dropper vertically and transfer 1 drop of serum or plasma (approximately 25 mL) to the specimen well (S) of the Test Cassette, and add 1 drop of buffer (approximately 55 mL), then start the timer. See illustration below.

For Venipuncture Whole Blood specimens:

Hold the dropper vertically and transfer 2 drops of whole blood (approximately 50 mL) to the specimen well (S) of the Test Cassette, and add 1 drop of buffer (approximately 55 mL), then start the timer. See illustration below.

For Fingerstick Whole Blood specimens:

To use a capillary tube: Fill the capillary tube and transfer approximately 50 mL of fingerstick whole blood specimen to the specimen well (S) of the Test Cassette, then add 1 drop of buffer (approximately 55 mL) and start the timer. See illustration below.

     3.Wait for the colored line(s) to appear. Read results at 5 minutes. Do not interpret the result after 10 minutes.

INTERPRETATION OF RESULTS

INTERPRETATION OF RESULTS

(Please refer to the illustration above)

POSITIVE:* Two distinct colored lines appear. One line should be in the control line region (C) and another line should be in the test line region (T).

*NOTE: The intensity of the color in the test line region (T) will vary depending on the concentration of IM heterophile antibodies present in the specimen. Therefore, any shade of color in the test line region (T) should be considered positive.

NEGATIVE: One colored line appears in the control line region (C). No apparent colored line appears in the test line region (T).

INVALID: Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test with a new test. If the problem persists, discontinue using the test kit immediately and contact your local distributor.