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High sensitivity Giardia lamblia Rapid Test Cassette , lateral flow test strips

Basic Information
Place of Origin: CHINA
Brand Name: AllTest
Certification: CE
Model Number: IGL-602
Minimum Order Quantity: 500
Packaging Details: 10T/Kit
Supply Ability: 100 Million a year
Format: Cassette Specimen: Feces
Kit Size: 10T/Kit Cut-Off: See Insert
Storage: 2-30℃ Shelf Time: 24 Months
High Light:

rapid test kit

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diagnostic tests for infection

A rapid test for the qualitative detection of Giardia lamblia in human stool samples CE certified

 

 

Applications:

 

The Giardia Lamblia Rapid Test Cassette (Feces) is a rapid chromatographic immunoassay for the qualitative detection of Giardia Lamblia in human feces specimen.

 

 

Description:

 

Parasitary infections remain a very serious health problem worldwide. Giardia lamblia is the most common protozoa known to be responsible for one of the main causes of severe diarrhoea in humans, particularly in immunodepressed people. Epidemiological studies, in 1991, showed that infections with Giardia increased in the United States with a prevalence of around 6% on 178,000 samples. Generally, the disease passes through a short acute phase followed by a chronic phase. Infection by G. lamblia, in the acute phase, is the cause of watery diarrhoea with principally the elimination of trophozoites. The stools become normal again, during the chronic phase, with transient emissions of cysts.

The presence of the parasite on the wall of the duodenal epithelium is responsible for a malabsorption. The disappearance of villosities and their atrophy lead to problems with the digestive process at the level of the duodenum and the jejunum, followed by weight loss and dehydration. The majority of infections remain asymptomatic, however.

The diagnosis of G. lamblia is carried out under microscopy after flotation on zinc sulphate or by direct or indirect immunofluorescence, on non-concentrated samples displayed on a slide. More and more ELISA methods are also now available for the specific detection of cysts and/or trophozoïtes. Detection of this parasite in surface or distribution water can be undertaken by PCR type techniques.

Coris BioConcept has developed a rapid membrane test that can detect Giardia lamblia in non-concentrated faecal samples within 15 minutes. The test is based on the detection of a 65-kDA coproantigen, a glycoprotein that is present in the cysts and trophozoites of G. lamblia.

 

 

 

How to use?

 

  1.To collect fecal specimens:

Collect sufficient quantity of feces (1-2mL or 1-2g) in a clean, dry specimen collection container to obtain enough virus particles. Best results will be obtained if the assay is performed within 6 hours after collection. Specimen collected may be stored for 3 days at 2-8°C if not tested within 6 hours. For long term storage, specimens should be kept below -20°C.

 

  2. To process fecal specimens:

Unscrew the cap of the specimen collection tube, then randomly stab the specimen collection applicator into the fecal specimen at least 3 different sites to collect approximately 50 mg of feces (equivalent to 1/4 of a pea). Do not scoop the fecal specimen.

Hold the dropper vertically, aspirate fecal specimens, and then transfer 2 drops of the liquid specimen (approximately 80 µL) into the specimen collection tube containing the extraction buffer.

Tighten the cap onto the specimen collection tube, then shake the specimen collection tube vigorously to mix the specimen and the extraction buffer. Leave the collection tube for reaction for 2 minutes.

 

  3.Bring the pouch to room temperature before opening it. Remove the test cassette from the foil pouch and use it as soon as possible. Best results will be obtained if the test is performed immediately after opening the foil pouch.

 

  4.Hold the specimen collection tube upright and unscrew the tip of the specimen collection tube. Invert the specimen collection tube and transfer 3 full drops of the extracted specimen (approximately 120 mL) to the specimen well (S) of the test cassette, then start the timer. Avoid trapping air bubbles in the specimen well (S). See illustration below.

 

  5.Read the results at 15 minutes after dispensing the specimen. Do not read results after 20 minutes.

Note: If the specimen does not migrate (presence of particles), centrifuge the diluted sample contained in the extraction buffer vial. Collect 120 µL of supernatant, dispense into the specimen well (S). Start the timer and continue from step 5 onwards in the above instructions for use.

 

High sensitivity Giardia lamblia Rapid Test Cassette , lateral flow test strips 0

 

 

 

INTERPRETATION OF RESULTS

 

Negative test result: a reddish-purple line appears across the central reading window at the Control line (C) position. No other band is present.

Positive test result: in addition to a reddish-purple band at the Control line (C), a visible reddish-purple band appears at the Test line position (T). Intensity of the test line may vary according to the quantity of antigens found in the sample. Any reddish-purple line (T), even weak, should be considered as a positive result.

Invalid test result: The absence of a Control line indicates a failure in the test procedure. Repeat invalid tests with a new test device.

Note: during the drying process, a very faint shadow may appear at the Test line position. It should not be regarded as a positive result.

 

Contact Details
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Phone Number : +8615857153722

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