The Rabies Virus Antigen Rapid Test Cassette (Saliva/Serum/Plasma) is a lateral flow immunochromatographic assay for the qualitative of Rabies Virus Antigen in canine’s saliva, serum or plasma.
PRINCIPLE
Rabies Virus Antigen Rapid Test Cassette is based on lateral flow immunochromatographic assay. The test cassette has a testing window. The testing window has an invisible T (test) zone and C (control) zone. When sample is applied into the sample well on the cassette, the liquid will laterally flow on the surface of the test strip. If there is enough rabies virus antigen in the sample, a visible T line will appear. The C line should always appear after a sample is applied, indicating a valid result. By this means, the cassette can accurately indicate the presence of rabies virus antigen in the sample.
STORAGE AND STABILITY
Store as packaged in the sealed pouch either at room temperature or refrigerated (2-30 °C). The test cassette is stable through the expiration date printed on the sealed pouch. The test cassette must remain in the sealed pouch until use. DO NOT FREEZE. Do not store the test kit in direct sunlight.
PRECAUTIONS
Do not use after expiration date.
Handle all specimens as if they contain infectious agents.
Observe established precautions against microbiological hazards throughout testing and follow the standard procedures for proper disposal of specimens.
Wear disposable gloves and eye protection when specimens are being tested.
Humidity and temperature can adversely affect results.
Do not remove test cassette from its pouch until immediately before use.
Do not reuse the test kit.
Do not mix components from different lot and different products.
MATERIALS
Materials Provided
• Test Cassettes
• Droppers
• Sterile swabs
• Buffer
• Package Insert
Materials Required But Not Provided
• Specimen collection containers • Centrifuge
• Timer
DIRECTIONS FOR USE
Allow the test cassette, specimen, buffer to equilibrate to room temperature (15-30 °C) prior to testing.
1.
Remove the test cassette from the foil pouch and use it as soon as possible. Best results will be obtained if the assay is
performed within one hour.
For saliva swab Specimens:
Collect dog’s saliva with the swab provided. Make the swab wet sufficiently. Insert the wet swab into the provided assay buffer tube. Agitate it to assure good sample extraction.
For serum/plasma Specimens:
Collect canine’s fresh whole blood, or separate serum or plasma from blood as soon as possible to avoid hemolysis. Only use clear, non-hemolyzed specimens. Do not leave the specimens at room temperature for prolonged periods. Serum and plasma specimens may be
stored at 2-8 °C for up to 3 days. For long term storage, specimens should be kept below -20 °C. Hold the dropper vertically and transfer 2 drop of serum/plasma (approximately 80 μL) into the extraction buffer and mix it up.
2.
Place the test cassette on a clean and level surface. Hold the dropper vertically and transfer 3 drops of extracted sample (approximately 120 μl) to the specimen well (S) of the test cassette, then start the timer. See illustration below.
3.
Read the results in 10 minutes by comparing the T line intensity with the color card provided. Do not interpret results after 15 minutes.
