For professional in vitro diagnostic use only.
The Etomidate (ETO) Rapid Test Dipstick (Urine) is a rapid chromatographic immunoassay for the detection of N,N-Dimethyltryptamine in urine at a cut-off concentration of 1,000 ng/mL. This test will detect other related compounds, please refer to the Analytical Specificity table in this
This assay provides only a qualitative, preliminary test result. A more specific alternate chemical method must be used in order to obtain a confirmed analytical result. Gas chromatography/mass spectrometry (GC/MS) is the preferred confirmatory method. Clinical consideration and
professional judgment should be applied to any drug of abuse test result, particularly when preliminary positive results are used.
Etomidate is a anesthetic drug that is often used in combination with other anesthetic drugs during surgery. Since 2011, the misuse or abuse of etomidate has gradually increased when propofol was designated a controlled drug under the Narcotics Control Act in Korea. The major
adverse effects of etomidate include adrenocortical suppression and unproven associated deaths, as well as myoclonus requiring pre-treatment. Although the issue of abuse and misuse of etomidate is emerging in recent years, there are few academic reports on these issues and
analytical methods in the forensic field. In order to effectively manage the misuse or abuse of etomidate, it is necessary to continuously monitor related cases with great interest and to be more intensively studied on its abuse potential.1
Etomidate is one of the popular rapidly acting inducing agents with cardio-stable profile but associated with side effects such as myoclonus. Myoclonus can lead to regurgitation, prolapse of vitreous in open globe or many other complications.2
The Etomidate (ETO) Rapid Test Dipstick (Urine) is a rapid urine-screening test that can be performed without the use of an instrument. The test utilizes the antibody to selectively detect elevated levels of Etomidate in urine. The Etomidate (ETO) Rapid Test Dipstick (Urine) yields a positive result when the Etomidate in urine exceed the cut-off level.
The Etomidate (ETO) Rapid Test Dipstick (Urine) is an immunoassay based on the principle of competitive binding. Drugs which may be present in the urine specimen compete against the drug conjugate for binding sites on the antibody.
During testing, a urine specimen migrates upward by capillary action. Etomidate, if present in the urine specimen below the cut-off level, will not saturate the binding sites of the antibody in the test. The antibody coated particles will then be captured by immobilized Etomidate-protein
conjugate and a visible colored line will show up in the test line region. The colored line will not form in the test line region if the Etomidate level exceeds the cut-off level, because it will saturate all the binding sites of anti-Etomidate antibody.
A drug-positive urine specimen will not generate a colored line in the test line region because of drug competition, while a drug-negative urine specimen or a specimen containing a drug concentration less than the cut-off will generate a line in the test line region. To serve as a procedural control, a colored line will always appear at the control line region indicating that proper volume of specimen has been added and membrane wicking has occurred.